Abstract 402: The role of IL-6 in communication of pancreatic carcinoma cells with fibroblasts

• Published in: Cancer research (Chicago, Ill.) Ill.), 2011-04, Vol.71 (8_Supplement), p.402-402
• Main Authors: Kamohara, Hidenobu, Kinoshita, Kouichi, Kurashige, Junji, Tanaka, Youhei, Baba, Hideo
• Format: Article
• Language: English
• ISSN: 0008-5472; 1538-7445
• DOI: 10.1158/1538-7445.AM2011-402

Pancreatic cancer shows high mortality rates in gastroenterogical neoplasms, and there are difficulties in early diagnosis and aggressive progression. IL-6 has pleiotropic function and are produced by various immunnocompetent cells, as well as cancer cells. Some studies have been demonstrated IL-6 play an important role in evading host immune surveillance in tumor microenvironment, but interactions of fibroblasts has not been fully understood. Therefore, the aim of this study is to reveal role of fibroblasts in pancreatic tumor microenvironment. Especially, we evaluate induction and biologicial function of IL-6 in pancreatic carcinoma cells after the stimulation with various cytokines and fibroblast associated factors. Firstly, we examined IL-6 and its receptors expression by RT-PCR and Northern blotting. All 7 tested cell lines expressed gp130 and IL-6R alpha mRNA, 2 cell lines (Hs7667 and Capan1) expressed IL-6 mRNA in serum free condition. To clarify the effect of proinflammatory cytokines on IL-6 expression in Hs766T cells, cells were stimulated with or without cytokines. Northern blotting revealed TNFα and IL-1β upregulated IL-6 mRNA, but not IL-6, IL-8 and LIF. IL-6 did not affect cell proliferation by WTS assay in 7 pancreatic carcinoma cells. In contrast, IL-6 promoted cell motility significantly by gold particles phagocytosis assay (P