Abstract 585: Eicosapentaenoic acid increases mammary tumor inhibition of intermittent calorie restriction and regulates adipokines

• Published in: Cancer research (Chicago, Ill.) Ill.), 2012-04, Vol.72 (8_Supplement), p.585-585
• Main Authors: Mizuno, Nancy K., Rogozina, Olga P., Seppanen, Christine M., Liao, D. Joshua, Cleary, Margot P., Grossmann, Michael E.
• Format: Article
• Language: English
• ISSN: 0008-5472; 1538-7445
• DOI: 10.1158/1538-7445.AM2012-585

It has become clear that the use of combination therapies for prevention of breast cancer may be more efficacious than individual therapies. Therefore, we combined two different interventions which had each been shown alone to inhibit mammary tumorigenesis but had not been tested in combination. The first intervention was consumption of the omega-3 fatty acid eicosapentaenoic acid (EPA) which is found in fish oil and the second intervention was calorie restriction. We utilized six groups of MMTV-Her2/neu mice as a model for human breast cancers which over express Her2/neu. Starting at 10 weeks of age half of the mice were fed a control diet composed of a modified AIN-93M formula with fat derived from soy oil. The other half consumed a diet with 71.75% of fat calories from EPA. Mice were further divided into ad libitum (AL), chronic calorie restricted (CCR) or intermittent caloric restricted (ICR) groups. AL groups (Control and EPA) received unrestricted access to their diets. CCR groups were given 75% of the total calories that the AL age-matched groups consumed. ICR groups were fed calories equal to 100% of the AL age-matched groups for three weeks followed by three weeks of calories equal to 50% of the AL age-matched groups. The 6 week cycles of restriction and refeeding were maintained until the mice were 60 weeks of age or were euthanized due to mammary tumors (MTs). MT incidence was lowest in the ICR-EPA group (15%) and highest in the AL-Con group (87%) while the AL-EPA, CCR-Con, CCR-EPA and ICR-Con had MT incidence rates of 63%, 47%, 40% and 59%, respectively The average survival until terminal end point was also longest in the ICR-EPA group at 59.2 weeks and shortest in the AL-Con group at 52.6 (P