Abstract 3086: Modulation of microRNA profile in lung adenocarcinoma cell line following TIMP-1 downregulation

Non-small cell carcinoma accounts for 85% of lung cancer cases and has a 5 year survival rate of only 17%. In recent years, tissue inhibitor of metalloproteinase-1 (TIMP-1) has emerged as an important prognostic marker. In several cancers including non-small cell lung cancer, high serum levels of TI...

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Published in:Cancer research (Chicago, Ill.) Ill.), 2013-04, Vol.73 (8_Supplement), p.3086-3086
Main Authors: Ghoshal-Gupta, Sampa, Kutiyanawalla, Ammar, Jiwani, Shahanawaz, Ojha, Juhi, Kolhe, Ravindra, Rojiani, Amyn M., Rojiani, Mumtaz V.
Format: Article
Language:English
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Summary:Non-small cell carcinoma accounts for 85% of lung cancer cases and has a 5 year survival rate of only 17%. In recent years, tissue inhibitor of metalloproteinase-1 (TIMP-1) has emerged as an important prognostic marker. In several cancers including non-small cell lung cancer, high serum levels of TIMP-1 have been associated with poor prognosis. Previously, we have shown that overexpressing TIMP-1 in H2009, a lung adenocarcinoma cell line resulted in aggressive tumors when implanted in the mouse brain. In the present study, we have knocked down TIMP-1 expression in the non-small cell lung cancer cell line A549 using shRNA. In vitro assays using the TIMP-1 knockdown clones defined decreased migration in wound assays (p
ISSN:0008-5472
1538-7445
DOI:10.1158/1538-7445.AM2013-3086