Abstract 5344: IGF1-receptor expression is regulated by miR-100 in metastatic pancreatic cancer cells

• Published in: Cancer research (Chicago, Ill.) Ill.), 2013-04, Vol.73 (8_Supplement), p.5344-5344
• Main Authors: Huang, Justin S., Egger, Michael, Grizzle, William E., McNally, Lacey R.
• Format: Article
• Language: English
• ISSN: 0008-5472; 1538-7445
• DOI: 10.1158/1538-7445.AM2013-5344

Patients with pancreatic adenocarcinoma have the lowest 5-year survival and yearly rates of incidence are almost equivalent to the mortality rates. Long-term cure rates by standard therapies are disappointing due to disseminated disease at diagnosis and chemotherapeutic resistance. New therapeutic targets are necessary for decreasing the progression of pancreatic cancer and identifying targets specific to metastasis would improve patient care. In this study, we evaluated the levels of microRNA of metastatic and non-metastatic cell lines. The expression level of microRNAs and mRNAs were identified through microarray analysis by examining and comparing 5 pancreatic cancer cell lines, two that can metastasize in vivo (S2VP10, S2CP9) versus three that do not metastasize (MiaPaCa2, Panc-1, and ASPC-1). MicroRNA analysis indicated an increase in miR-100 and a decrease in miR-138 expression in metastatic cancer cells. Microarray analysis of differentially expressed mRNAs between metastatic and non-metastatic pancreatic cell lines also indicated increased insulin growth factor-1 receptor (IGF1-R) expression (p