Abstract LB-356: Catalase and NADPH oxidase single nucleotide polymorphisms are associated with increased risk and serve as potential targets for breast and ovarian cancers

• Published in: Cancer research (Chicago, Ill.) Ill.), 2013-04, Vol.73 (8_Supplement), p.LB-356-LB-356
• Main Authors: Belotte, Jimmy, Fletcher, Nicole M., Levin, Nancy K., Simon, Michael S., Abu-Soud, Husam M., Diamond, Michael P., Tainsky, Michael A., Saed, Ghassan M.
• Format: Article
• Language: English
• ISSN: 0008-5472; 1538-7445
• DOI: 10.1158/1538-7445.AM2013-LB-356

We investigated the association of a SNP in catalase (CAT) and in the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase p22-phox subunit with an increased risk of development of ovarian and breast cancers. Both enzymes play major roles in the regulation of the redox balance. In the general population, the minor allele frequency (MAF) for CAT SNP C-262T (rs1001179) is 12.4% and 30.3%for NADPH oxidase SNP C242T (rs4673). The risk of breast cancer has been associated with decreased activity of CAT, which was attributed to this specific SNP. Similarly, an increase in NADPH oxidase activity has been associated with specific SNP in the CYBA gene, encoding the p22-phox subunit of NAPDH oxidase, substituting allele C with T at position 242 located on chromosome 16q24. Patients (n=190) were recruited from the Karmanos Cancer Institute, Detroit, MI for genetic counseling based on personal and family histories. Stored blood samples were subjected to DNA extraction (QIAamp DNA mini kit) followed by TaqMan® SNP Genotyping Assay Sets for rs1001179 or rs4673 run on the QuantStudio™ 12K Flex Real-Time PCR System. Data were analyzed using SPSS for Mac V.21, utilizing Chi-squared and regression analysis with p