Abstract 5225: MicroRNA profiling in serum samples from donors with germ cell cancer

• Published in: Cancer research (Chicago, Ill.) Ill.), 2014-10, Vol.74 (19_Supplement), p.5225-5225
• Main Authors: Lee, Kathy Y., Patel, Sunali, Hayashibara, Kathleen, Chu, Shirley, Gillis, Ad J. M, Rijlaarsdam, Martin, Dorssers, Lambert C.J, Looijenga, Leendert H. J
• Format: Article
• Language: English
• ISSN: 0008-5472; 1538-7445
• DOI: 10.1158/1538-7445.AM2014-5225

MicroRNAs are short non-coding RNA molecules (∼21 bases) that have been identified as important regulators of gene expression at the translational and transcriptional level. They are known to play a crucial role in cell development, differentiation, and disease. Dysregulation of miRNAs has been linked to cancer development and tumor progression. In addition, miRNAs have been identified as tumor classifiers and disease biomarkers. Recent studies have shown that miRNAs are present in body fluids (serum, saliva, semen, urine) thus providing a non-invasive tool to study and monitor disease states. Earlier research studies identified specific miRNAs as characteristic for germ cell tumors, such as testicular cancer (miR-372, miR-373). miRNA Profiling (∼760 miRNAs) was performed to identify additional miRNAs as candidate biomarkers in serum samples for testicular cancer (seminoma and non-seminoma types), from normal and cancer tissue in parallel with their matched serum samples collected from patients with testicular cancer. For this research study we used a miRNA specific bead capture system to isolate miRNAs from serum and TaqMan® Array Card platform for profiling. We will present results from this research study comparing the miRNA expression patterns in matched normal, cancer tissue, and serum. For Research Use Only. Not for use in diagnostic procedures. Citation Format: Kathy Y. Lee, Sunali Patel, Kathleen Hayashibara, Shirley Chu, Ad J. M Gillis, Martin Rijlaarsdam, Lambert C.J Dorssers, Leendert H. J Looijenga. MicroRNA profiling in serum samples from donors with germ cell cancer. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 5225. doi:10.1158/1538-7445.AM2014-5225