Abstract 4277: Oral administration of a traditional herbal remedy, JC001, suppresses pancreatic tumorigenesis in mice via regulating immune cell functions

• Published in: Cancer research (Chicago, Ill.) Ill.), 2015-08, Vol.75 (15_Supplement), p.4277-4277
• Main Authors: Jan, Ming-Shiou, Hsu, Fong-Rong, Lin, You-Chain, Chuang, Shu-Han, Lin, Jia-Wei, Chuang, Meng-Hsein, Hsu, Li-Jin
• Format: Article
• Language: English
• ISSN: 0008-5472; 1538-7445
• DOI: 10.1158/1538-7445.AM2015-4277

The traditional Chinese herbal medicine JC001 is widely used as an effective liver-protecting remedy in Taiwan and China for decades. We have first examined the antitumor effects of JC001 using both mouse xenograft and oncogene-induced tumor models. Our results indicated that the numbers of M1-type macrophages, mitogen-stimulated T cell proliferation and natural killer cell activity were increased in wild-type mice with oral administration of JC001. Interestingly, continuous administration of JC001 for a month markedly reduced the numbers and the sizes of tumor nodules in a mouse pancreatic xenograft model. In contrast to this observation in immune-competent mice, our data revealed that the average tumor size in JC001-administrated nude mice was comparable with that of the control tumor-bearing nude mice without JC001 treatment, suggesting that JC001-induced antitumor activity is mediated by T cells. Adoptive transfer of the splenocytes isolated from immune-competent mice with JC001 treatment into tumor-bearing nude mice reduced tumor growth in these T cell-deficient mice. Moreover, increased cytotoxic activity of natural killer (NK) cells was also observed in mice treated with JC001. In an oncogene-induced tumor model, orally administered JC001 significantly suppressed caerulein-triggered development of pancreatic neoplasias and cancer cell metastasis in KrasG12D/w Pdx-Cre transgenic mice. Together, our results clearly suggest that JC001 exerts immunomodulatory activity mainly through regulating T and NK cell responses to suppress pancreatic cancer development and metastasis. Citation Format: Ming-Shiou Jan, Fong-Rong Hsu, You-Chain Lin, Shu-Han Chuang, Jia-Wei Lin, Meng-Hsein Chuang, Li-Jin Hsu. Oral administration of a traditional herbal remedy, JC001, suppresses pancreatic tumorigenesis in mice via regulating immune cell functions. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 4277. doi:10.1158/1538-7445.AM2015-4277