Abstract 2218: Quantitative multiplex analysis of immune checkpoint protein expression in circulation and in the tumor microenvironment

Immune checkpoint inhibitors have been proven to be an effective method in improving antitumor immune response. Many immune checkpoint proteins are expressed as soluble forms in circulation and in the tumor and tumor microenvironment. Here we report the development of bead-based Luminex multiplex as...

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Published in:Cancer research (Chicago, Ill.) Ill.), 2017-07, Vol.77 (13_Supplement), p.2218-2218
Main Authors: Lie, Wen-Rong, Mistry, Jehangir
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Mistry, Jehangir
description Immune checkpoint inhibitors have been proven to be an effective method in improving antitumor immune response. Many immune checkpoint proteins are expressed as soluble forms in circulation and in the tumor and tumor microenvironment. Here we report the development of bead-based Luminex multiplex assays for the quantitative profiling of co-inhibitory and co-stimulating immune checkpoint proteins CTLA-4, PD-1, TIM-3, LAG-3, HVEM. GITRL, BTLA, CD27, CD28, CD40, GITR, PD-L1, B7-1/CD80, B7-2/CD86, and ICOS. In order to explore the use of soluble immune checkpoint proteins as putative cancer biomarkers, we used these multiplex assays to measure checkpoint protein levels in serum samples from breast cancer patients, colon cancer patients, and a corresponding set of normal serum samples. Analysis of the soluble checkpoint protein signatures generated from this multiplex approach revealed a significantly elevated level of soluble TIM-3 protein in the breast cancer serum samples and in the colon cancer serum samples compared to the healthy serum controls (p
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Many immune checkpoint proteins are expressed as soluble forms in circulation and in the tumor and tumor microenvironment. Here we report the development of bead-based Luminex multiplex assays for the quantitative profiling of co-inhibitory and co-stimulating immune checkpoint proteins CTLA-4, PD-1, TIM-3, LAG-3, HVEM. GITRL, BTLA, CD27, CD28, CD40, GITR, PD-L1, B7-1/CD80, B7-2/CD86, and ICOS. In order to explore the use of soluble immune checkpoint proteins as putative cancer biomarkers, we used these multiplex assays to measure checkpoint protein levels in serum samples from breast cancer patients, colon cancer patients, and a corresponding set of normal serum samples. Analysis of the soluble checkpoint protein signatures generated from this multiplex approach revealed a significantly elevated level of soluble TIM-3 protein in the breast cancer serum samples and in the colon cancer serum samples compared to the healthy serum controls (p&lt;0.001). In addition, we analyzed the immune checkpoint protein expression profiles in lysates of tumor and adjacent normal tissues from 3 patients with metastatic breast cancer and 2 patients with colorectal cancer. Differential expression of multiple checkpoint proteins, including BTLA, CD27, TIM-3, HVEM, CD40, GITR, LAG3, CTLA-4, B7-1, PD-L1, or ICOS, were detected in these matched lysates, indicating the roles and complexity of checkpoint proteins in tumor microenvironment. In summary, our results demonstrate that the multiplex assays we developed are useful research tools for the simultaneously quantitation of immune checkpoint proteins, as well as its potential application in cancer biomarker discovery and translational research in cancer immunotherapy. Citation Format: Wen-Rong Lie, Jehangir Mistry. Quantitative multiplex analysis of immune checkpoint protein expression in circulation and in the tumor microenvironment [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. 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In addition, we analyzed the immune checkpoint protein expression profiles in lysates of tumor and adjacent normal tissues from 3 patients with metastatic breast cancer and 2 patients with colorectal cancer. Differential expression of multiple checkpoint proteins, including BTLA, CD27, TIM-3, HVEM, CD40, GITR, LAG3, CTLA-4, B7-1, PD-L1, or ICOS, were detected in these matched lysates, indicating the roles and complexity of checkpoint proteins in tumor microenvironment. In summary, our results demonstrate that the multiplex assays we developed are useful research tools for the simultaneously quantitation of immune checkpoint proteins, as well as its potential application in cancer biomarker discovery and translational research in cancer immunotherapy. Citation Format: Wen-Rong Lie, Jehangir Mistry. Quantitative multiplex analysis of immune checkpoint protein expression in circulation and in the tumor microenvironment [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. 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In addition, we analyzed the immune checkpoint protein expression profiles in lysates of tumor and adjacent normal tissues from 3 patients with metastatic breast cancer and 2 patients with colorectal cancer. Differential expression of multiple checkpoint proteins, including BTLA, CD27, TIM-3, HVEM, CD40, GITR, LAG3, CTLA-4, B7-1, PD-L1, or ICOS, were detected in these matched lysates, indicating the roles and complexity of checkpoint proteins in tumor microenvironment. In summary, our results demonstrate that the multiplex assays we developed are useful research tools for the simultaneously quantitation of immune checkpoint proteins, as well as its potential application in cancer biomarker discovery and translational research in cancer immunotherapy. Citation Format: Wen-Rong Lie, Jehangir Mistry. Quantitative multiplex analysis of immune checkpoint protein expression in circulation and in the tumor microenvironment [abstract]. 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title Abstract 2218: Quantitative multiplex analysis of immune checkpoint protein expression in circulation and in the tumor microenvironment
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