Loading…

Abstract 473: PRKRA/PACT expression promotes chemoresistance in mucinous ovarian cancer

Purpose: To investigate the mechanisms of chemotherapy resistance and developing strategies to enhance therapeutic responses in mucinous ovarian cancer (MOC). Experimental design: We carried out a kinome-based siRNA screen using human MOC to identify novel targets to enhance the efficacy of chemothe...

Full description

Saved in:
Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 2017-07, Vol.77 (13_Supplement), p.473-473
Main Authors: Hisamatsu, Takeshi, McGuire, Michael, Wu, Sherry Y., Rupaimoole, Rajesha, Pradeep, Sunila, Noh, Kyunghee, Filant, Justyna, Hansen, Jean M., Lyons, Yasmin, Gharpure, Kshipra M., Nagaraja, Archana S., Mangala, Lingegowda S., Mitamura, Takashi, Rodriguez-Aguayo, Cristian, Bartholomeusz, Geoffrey A., Ivan, Cristina, Lee, Ju-Seong, Matsuo, Koji, Frumovitz, Michael, Wong, Kwong K., Lopez-Berestein, Gabriel, Sood, Anil K.
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purpose: To investigate the mechanisms of chemotherapy resistance and developing strategies to enhance therapeutic responses in mucinous ovarian cancer (MOC). Experimental design: We carried out a kinome-based siRNA screen using human MOC to identify novel targets to enhance the efficacy of chemotherapy in MOC cell lines. In vitro and in vivo validation studies were carried out using MOC models. We specifically interrogated the role of PRKRA in MOC based on our screen results. Results: Among the 939 genes in the screen, we focused on PRKRA/PACT because it was one of the top 5 target genes that exhibited the greatest extent of synthetic lethality in the target gene-siRNA plus oxaliplatin group relative to the target gene-siRNA group. The combination of oxaliplatin plus siPRKRA treatment resulted in significantly reduced cell viability compared with oxaliplatin plus control siRNA in RMUG-L-ip1 or RMUG-S-ip1 MOC cells (p
ISSN:0008-5472
1538-7445
DOI:10.1158/1538-7445.AM2017-473