Abstract 1572: Single cell sequencing reveals the microenvironment network in multifocal pulmonary nodules

• Published in: Cancer research (Chicago, Ill.) Ill.), 2020-08, Vol.80 (16_Supplement), p.1572-1572
• Main Authors: Liu, Dongcheng, Pan, Pan, Li, Shixuan, Cheng, Zhiqiang, Wang, Guangsuo, Zou, Chang
• Format: Article
• Language: English
• ISSN: 0008-5472; 1538-7445
• DOI: 10.1158/1538-7445.AM2020-1572

Tumor microenvironment plays an important role in tumorigenesis in cancer development, however, little is known about its heterogeneity and evolution progression during early stage of lung cancer. In this study, single cell sequencing was applied to uncover the transcriptome in the microenvironment and the complex ecosystem of stromal cells in pulmonary nodules including atypical adenomatous hyperplasia (AAH) adenocarcinoma in situ (AIS), invasive adenocarcinoma (MIA) and invasive adenocarcinoma (IA). Although the expression or mutation of driver genes were detected in different stage of pulmonary lesion tissues that might contribute to the development of lung cancer, tumor environment including immune system are found to be important for the progression of tumorigeneses. This study provided evidence that the different evolutionary selection and microenvironment network in multifocal pulmonary nodules in the same individual resulted in various cancer progression stages. To our knowledge, this study first provides an overview of the genetic basis and tumor environment of human lung nodule by single-cell sequencing. Keywords: Single cell sequencing; heterogeneous; microenvironment; multifocal pulmonary nodules Citation Format: Dongcheng Liu, Pan Pan, Shixuan Li, Zhiqiang Cheng, Guangsuo Wang, Chang Zou. Single cell sequencing reveals the microenvironment network in multifocal pulmonary nodules [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 1572.