Loading…

Abstract 2212: Racially disparate serum levels of inflammatory cytokines, satiety and stress hormones, and exosomal microRNAs in women with or without a breast cancer diagnosis

Breast cancer (BC) is the most common non-cutaneous malignancy and the second leading cause of cancer-related death in American women. Health disparities in incidence and clinical outcome are also reported among different racial groups, most notably African American (AA) women, who are often diagnos...

Full description

Saved in:
Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 2022-06, Vol.82 (12_Supplement), p.2212-2212
Main Authors: Sudan, Sarabjeet Kour, Vikramdeo, Kunwar Somesh, Sharma, Amod, Deshmukh, Sachin Kumar, Srivastava, Sanjeev Kumar, Poosarla, Teja, Holliday, Nicolette P., Dyess, Donna L., Singh, Ajay P., Singh, Seema
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 2212
container_issue 12_Supplement
container_start_page 2212
container_title Cancer research (Chicago, Ill.)
container_volume 82
creator Sudan, Sarabjeet Kour
Vikramdeo, Kunwar Somesh
Sharma, Amod
Deshmukh, Sachin Kumar
Srivastava, Sanjeev Kumar
Poosarla, Teja
Holliday, Nicolette P.
Dyess, Donna L.
Singh, Ajay P.
Singh, Seema
description Breast cancer (BC) is the most common non-cutaneous malignancy and the second leading cause of cancer-related death in American women. Health disparities in incidence and clinical outcome are also reported among different racial groups, most notably African American (AA) women, who are often diagnosed at a young age with aggressive BC and exhibit greater mortality than Caucasian American (CA) women. Since socioeconomic difficulties can have tremendous impact on psychophysiology besides limiting the access to optimal healthcare, we examined the serum levels of stress (cortisol) and satiety (leptin) hormones as well as inflammatory cytokines (resistin and interleukin-6/IL-6) in AA and CA women. To observe a potential epigenomic connection, we also performed profiling of a targeted set of exosomal microRNAs in serum samples. The study was conducted under an Institutional Review Board (IRB) approved protocol. All subjects participated voluntarily, and their consents were obtained. Serum levels of resistin, IL-6, leptin, and cortisol were quantified by Enzyme linked Immunosorbent Assay (ELISA) using commercial kits. Exosomes were isolated using precipitation method and recovered by standard centrifugation. Total RNA was isolated from exosomal preps and subjected to stem-loop RT-PCR for quantitation of a set of inflammation-associated microRNAs. We found that the levels of resistin, leptin, IL-6 and cortisol were higher in women with a BC diagnosis than non-BC subjects. Moreover, AA women with or without BC showed significantly higher levels of these cytokines and hormones in their serum as compared to the CA women with or without a BC diagnosis, respectively. We also observed differential expression of several microRNAs in serum of BC women as compared to their normal counterparts, of which five (miR511, miR33a, miR27a, miR6794, miR143-3p) exhibited highest presence in serum exosomes of AA women with BC. Together, these findings suggest that relatively greater exposure of minority women to social stressors may have epigenomic consequences and may potentially be linked to the observed BC racial health disparities. Citation Format: Sarabjeet Kour Sudan, Kunwar Somesh Vikramdeo, Amod Sharma, Sachin Kumar Deshmukh, Sanjeev Kumar Srivastava, Teja Poosarla, Nicolette P. Holliday, Donna L. Dyess, Ajay P. Singh, Seema Singh. Racially disparate serum levels of inflammatory cytokines, satiety and stress hormones, and exosomal microRNAs in women with or without a breast c
doi_str_mv 10.1158/1538-7445.AM2022-2212
format article
fullrecord <record><control><sourceid>crossref</sourceid><recordid>TN_cdi_crossref_primary_10_1158_1538_7445_AM2022_2212</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_1158_1538_7445_AM2022_2212</sourcerecordid><originalsourceid>FETCH-crossref_primary_10_1158_1538_7445_AM2022_22123</originalsourceid><addsrcrecordid>eNqdUE1LQzEQDKJg_fgJwv4AX03SBou3IooXeyjewzbdZ6PJS8mm6vtX_kRfqohnLzvLLDPsjBAXSo6VMrMrZSaz5no6NeP5o5ZaN1orfSBGv_zhn_1YnDC_SCmNkmYkPucrLhldgSq6gSU6jyH0sPa8xYyFgCnvIgR6o8CQWvBdGzBGLCn34PqSXn1HfAmMxVPpAbs1DJ7EDJuUY9ofK0kfiVPEANG7nJaLOQ9e8J4iDdOXDaS8x7QrgLDKhFzAYecoD-_gc5fY85k4ajEwnf_gqTD3d0-3D81gyZyptdvsI-beKmlrPbZGtzW6_a7H1qST_-q-AGCmclg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Abstract 2212: Racially disparate serum levels of inflammatory cytokines, satiety and stress hormones, and exosomal microRNAs in women with or without a breast cancer diagnosis</title><source>EZB Electronic Journals Library</source><creator>Sudan, Sarabjeet Kour ; Vikramdeo, Kunwar Somesh ; Sharma, Amod ; Deshmukh, Sachin Kumar ; Srivastava, Sanjeev Kumar ; Poosarla, Teja ; Holliday, Nicolette P. ; Dyess, Donna L. ; Singh, Ajay P. ; Singh, Seema</creator><creatorcontrib>Sudan, Sarabjeet Kour ; Vikramdeo, Kunwar Somesh ; Sharma, Amod ; Deshmukh, Sachin Kumar ; Srivastava, Sanjeev Kumar ; Poosarla, Teja ; Holliday, Nicolette P. ; Dyess, Donna L. ; Singh, Ajay P. ; Singh, Seema</creatorcontrib><description>Breast cancer (BC) is the most common non-cutaneous malignancy and the second leading cause of cancer-related death in American women. Health disparities in incidence and clinical outcome are also reported among different racial groups, most notably African American (AA) women, who are often diagnosed at a young age with aggressive BC and exhibit greater mortality than Caucasian American (CA) women. Since socioeconomic difficulties can have tremendous impact on psychophysiology besides limiting the access to optimal healthcare, we examined the serum levels of stress (cortisol) and satiety (leptin) hormones as well as inflammatory cytokines (resistin and interleukin-6/IL-6) in AA and CA women. To observe a potential epigenomic connection, we also performed profiling of a targeted set of exosomal microRNAs in serum samples. The study was conducted under an Institutional Review Board (IRB) approved protocol. All subjects participated voluntarily, and their consents were obtained. Serum levels of resistin, IL-6, leptin, and cortisol were quantified by Enzyme linked Immunosorbent Assay (ELISA) using commercial kits. Exosomes were isolated using precipitation method and recovered by standard centrifugation. Total RNA was isolated from exosomal preps and subjected to stem-loop RT-PCR for quantitation of a set of inflammation-associated microRNAs. We found that the levels of resistin, leptin, IL-6 and cortisol were higher in women with a BC diagnosis than non-BC subjects. Moreover, AA women with or without BC showed significantly higher levels of these cytokines and hormones in their serum as compared to the CA women with or without a BC diagnosis, respectively. We also observed differential expression of several microRNAs in serum of BC women as compared to their normal counterparts, of which five (miR511, miR33a, miR27a, miR6794, miR143-3p) exhibited highest presence in serum exosomes of AA women with BC. Together, these findings suggest that relatively greater exposure of minority women to social stressors may have epigenomic consequences and may potentially be linked to the observed BC racial health disparities. Citation Format: Sarabjeet Kour Sudan, Kunwar Somesh Vikramdeo, Amod Sharma, Sachin Kumar Deshmukh, Sanjeev Kumar Srivastava, Teja Poosarla, Nicolette P. Holliday, Donna L. Dyess, Ajay P. Singh, Seema Singh. Racially disparate serum levels of inflammatory cytokines, satiety and stress hormones, and exosomal microRNAs in women with or without a breast cancer diagnosis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 2212.</description><identifier>ISSN: 1538-7445</identifier><identifier>EISSN: 1538-7445</identifier><identifier>DOI: 10.1158/1538-7445.AM2022-2212</identifier><language>eng</language><ispartof>Cancer research (Chicago, Ill.), 2022-06, Vol.82 (12_Supplement), p.2212-2212</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Sudan, Sarabjeet Kour</creatorcontrib><creatorcontrib>Vikramdeo, Kunwar Somesh</creatorcontrib><creatorcontrib>Sharma, Amod</creatorcontrib><creatorcontrib>Deshmukh, Sachin Kumar</creatorcontrib><creatorcontrib>Srivastava, Sanjeev Kumar</creatorcontrib><creatorcontrib>Poosarla, Teja</creatorcontrib><creatorcontrib>Holliday, Nicolette P.</creatorcontrib><creatorcontrib>Dyess, Donna L.</creatorcontrib><creatorcontrib>Singh, Ajay P.</creatorcontrib><creatorcontrib>Singh, Seema</creatorcontrib><title>Abstract 2212: Racially disparate serum levels of inflammatory cytokines, satiety and stress hormones, and exosomal microRNAs in women with or without a breast cancer diagnosis</title><title>Cancer research (Chicago, Ill.)</title><description>Breast cancer (BC) is the most common non-cutaneous malignancy and the second leading cause of cancer-related death in American women. Health disparities in incidence and clinical outcome are also reported among different racial groups, most notably African American (AA) women, who are often diagnosed at a young age with aggressive BC and exhibit greater mortality than Caucasian American (CA) women. Since socioeconomic difficulties can have tremendous impact on psychophysiology besides limiting the access to optimal healthcare, we examined the serum levels of stress (cortisol) and satiety (leptin) hormones as well as inflammatory cytokines (resistin and interleukin-6/IL-6) in AA and CA women. To observe a potential epigenomic connection, we also performed profiling of a targeted set of exosomal microRNAs in serum samples. The study was conducted under an Institutional Review Board (IRB) approved protocol. All subjects participated voluntarily, and their consents were obtained. Serum levels of resistin, IL-6, leptin, and cortisol were quantified by Enzyme linked Immunosorbent Assay (ELISA) using commercial kits. Exosomes were isolated using precipitation method and recovered by standard centrifugation. Total RNA was isolated from exosomal preps and subjected to stem-loop RT-PCR for quantitation of a set of inflammation-associated microRNAs. We found that the levels of resistin, leptin, IL-6 and cortisol were higher in women with a BC diagnosis than non-BC subjects. Moreover, AA women with or without BC showed significantly higher levels of these cytokines and hormones in their serum as compared to the CA women with or without a BC diagnosis, respectively. We also observed differential expression of several microRNAs in serum of BC women as compared to their normal counterparts, of which five (miR511, miR33a, miR27a, miR6794, miR143-3p) exhibited highest presence in serum exosomes of AA women with BC. Together, these findings suggest that relatively greater exposure of minority women to social stressors may have epigenomic consequences and may potentially be linked to the observed BC racial health disparities. Citation Format: Sarabjeet Kour Sudan, Kunwar Somesh Vikramdeo, Amod Sharma, Sachin Kumar Deshmukh, Sanjeev Kumar Srivastava, Teja Poosarla, Nicolette P. Holliday, Donna L. Dyess, Ajay P. Singh, Seema Singh. Racially disparate serum levels of inflammatory cytokines, satiety and stress hormones, and exosomal microRNAs in women with or without a breast cancer diagnosis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 2212.</description><issn>1538-7445</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqdUE1LQzEQDKJg_fgJwv4AX03SBou3IooXeyjewzbdZ6PJS8mm6vtX_kRfqohnLzvLLDPsjBAXSo6VMrMrZSaz5no6NeP5o5ZaN1orfSBGv_zhn_1YnDC_SCmNkmYkPucrLhldgSq6gSU6jyH0sPa8xYyFgCnvIgR6o8CQWvBdGzBGLCn34PqSXn1HfAmMxVPpAbs1DJ7EDJuUY9ofK0kfiVPEANG7nJaLOQ9e8J4iDdOXDaS8x7QrgLDKhFzAYecoD-_gc5fY85k4ajEwnf_gqTD3d0-3D81gyZyptdvsI-beKmlrPbZGtzW6_a7H1qST_-q-AGCmclg</recordid><startdate>20220615</startdate><enddate>20220615</enddate><creator>Sudan, Sarabjeet Kour</creator><creator>Vikramdeo, Kunwar Somesh</creator><creator>Sharma, Amod</creator><creator>Deshmukh, Sachin Kumar</creator><creator>Srivastava, Sanjeev Kumar</creator><creator>Poosarla, Teja</creator><creator>Holliday, Nicolette P.</creator><creator>Dyess, Donna L.</creator><creator>Singh, Ajay P.</creator><creator>Singh, Seema</creator><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20220615</creationdate><title>Abstract 2212: Racially disparate serum levels of inflammatory cytokines, satiety and stress hormones, and exosomal microRNAs in women with or without a breast cancer diagnosis</title><author>Sudan, Sarabjeet Kour ; Vikramdeo, Kunwar Somesh ; Sharma, Amod ; Deshmukh, Sachin Kumar ; Srivastava, Sanjeev Kumar ; Poosarla, Teja ; Holliday, Nicolette P. ; Dyess, Donna L. ; Singh, Ajay P. ; Singh, Seema</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-crossref_primary_10_1158_1538_7445_AM2022_22123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sudan, Sarabjeet Kour</creatorcontrib><creatorcontrib>Vikramdeo, Kunwar Somesh</creatorcontrib><creatorcontrib>Sharma, Amod</creatorcontrib><creatorcontrib>Deshmukh, Sachin Kumar</creatorcontrib><creatorcontrib>Srivastava, Sanjeev Kumar</creatorcontrib><creatorcontrib>Poosarla, Teja</creatorcontrib><creatorcontrib>Holliday, Nicolette P.</creatorcontrib><creatorcontrib>Dyess, Donna L.</creatorcontrib><creatorcontrib>Singh, Ajay P.</creatorcontrib><creatorcontrib>Singh, Seema</creatorcontrib><collection>CrossRef</collection><jtitle>Cancer research (Chicago, Ill.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sudan, Sarabjeet Kour</au><au>Vikramdeo, Kunwar Somesh</au><au>Sharma, Amod</au><au>Deshmukh, Sachin Kumar</au><au>Srivastava, Sanjeev Kumar</au><au>Poosarla, Teja</au><au>Holliday, Nicolette P.</au><au>Dyess, Donna L.</au><au>Singh, Ajay P.</au><au>Singh, Seema</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Abstract 2212: Racially disparate serum levels of inflammatory cytokines, satiety and stress hormones, and exosomal microRNAs in women with or without a breast cancer diagnosis</atitle><jtitle>Cancer research (Chicago, Ill.)</jtitle><date>2022-06-15</date><risdate>2022</risdate><volume>82</volume><issue>12_Supplement</issue><spage>2212</spage><epage>2212</epage><pages>2212-2212</pages><issn>1538-7445</issn><eissn>1538-7445</eissn><abstract>Breast cancer (BC) is the most common non-cutaneous malignancy and the second leading cause of cancer-related death in American women. Health disparities in incidence and clinical outcome are also reported among different racial groups, most notably African American (AA) women, who are often diagnosed at a young age with aggressive BC and exhibit greater mortality than Caucasian American (CA) women. Since socioeconomic difficulties can have tremendous impact on psychophysiology besides limiting the access to optimal healthcare, we examined the serum levels of stress (cortisol) and satiety (leptin) hormones as well as inflammatory cytokines (resistin and interleukin-6/IL-6) in AA and CA women. To observe a potential epigenomic connection, we also performed profiling of a targeted set of exosomal microRNAs in serum samples. The study was conducted under an Institutional Review Board (IRB) approved protocol. All subjects participated voluntarily, and their consents were obtained. Serum levels of resistin, IL-6, leptin, and cortisol were quantified by Enzyme linked Immunosorbent Assay (ELISA) using commercial kits. Exosomes were isolated using precipitation method and recovered by standard centrifugation. Total RNA was isolated from exosomal preps and subjected to stem-loop RT-PCR for quantitation of a set of inflammation-associated microRNAs. We found that the levels of resistin, leptin, IL-6 and cortisol were higher in women with a BC diagnosis than non-BC subjects. Moreover, AA women with or without BC showed significantly higher levels of these cytokines and hormones in their serum as compared to the CA women with or without a BC diagnosis, respectively. We also observed differential expression of several microRNAs in serum of BC women as compared to their normal counterparts, of which five (miR511, miR33a, miR27a, miR6794, miR143-3p) exhibited highest presence in serum exosomes of AA women with BC. Together, these findings suggest that relatively greater exposure of minority women to social stressors may have epigenomic consequences and may potentially be linked to the observed BC racial health disparities. Citation Format: Sarabjeet Kour Sudan, Kunwar Somesh Vikramdeo, Amod Sharma, Sachin Kumar Deshmukh, Sanjeev Kumar Srivastava, Teja Poosarla, Nicolette P. Holliday, Donna L. Dyess, Ajay P. Singh, Seema Singh. Racially disparate serum levels of inflammatory cytokines, satiety and stress hormones, and exosomal microRNAs in women with or without a breast cancer diagnosis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 2212.</abstract><doi>10.1158/1538-7445.AM2022-2212</doi></addata></record>
fulltext fulltext
identifier ISSN: 1538-7445
ispartof Cancer research (Chicago, Ill.), 2022-06, Vol.82 (12_Supplement), p.2212-2212
issn 1538-7445
1538-7445
language eng
recordid cdi_crossref_primary_10_1158_1538_7445_AM2022_2212
source EZB Electronic Journals Library
title Abstract 2212: Racially disparate serum levels of inflammatory cytokines, satiety and stress hormones, and exosomal microRNAs in women with or without a breast cancer diagnosis
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T08%3A03%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-crossref&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Abstract%202212:%20Racially%20disparate%20serum%20levels%20of%20inflammatory%20cytokines,%20satiety%20and%20stress%20hormones,%20and%20exosomal%20microRNAs%20in%20women%20with%20or%20without%20a%20breast%20cancer%20diagnosis&rft.jtitle=Cancer%20research%20(Chicago,%20Ill.)&rft.au=Sudan,%20Sarabjeet%20Kour&rft.date=2022-06-15&rft.volume=82&rft.issue=12_Supplement&rft.spage=2212&rft.epage=2212&rft.pages=2212-2212&rft.issn=1538-7445&rft.eissn=1538-7445&rft_id=info:doi/10.1158/1538-7445.AM2022-2212&rft_dat=%3Ccrossref%3E10_1158_1538_7445_AM2022_2212%3C/crossref%3E%3Cgrp_id%3Ecdi_FETCH-crossref_primary_10_1158_1538_7445_AM2022_22123%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true