Abstract 2531: a

• Published in: Cancer research (Chicago, Ill.) Ill.), 2022-06, Vol.82 (12_Supplement), p.2531-2531
• Main Authors: Rajesh, Adarsh, Havas, Aaron, Varanasi, Siva Karthik, Evans, Katrina, Cheikh, Bassem B., Duerrschmid, Clemens, Mammadova, Najiba, Adams, Peter, Kaech, Susan
• Format: Article
• Language: English
• ISSN: 1538-7445; 1538-7445
• DOI: 10.1158/1538-7445.AM2022-2531

Hepatocellular carcinoma (HCC) is an emerging health crisis due to rapidly increasing incidence rates and an overall 5-year survival rate of 18%. Consequently, there is an urgent need for new therapies and better preventive strategies. Aging and obesity are two of the biggest risk factors for HCC, and with ever-increasing aging and obese populations, uncovering how aging and obesity drive the increased risk for HCC has become imperative. Aging and obesity are well known to be characterized by immune dysregulation leading to persistent inflammation, and a dysregulated immune microenvironment is also a common theme shared by multiple hallmarks of HCC. In this study, we aim to assess the immune microenvironment of aged and obese mouse livers by performing spatial phenotyping of distinct immune signatures which can potentially predispose hepatocytes to HCC. To address this, we used Akoya’s ultra-high multiplexed CODEX (CO-Detection by indEXing) imaging system to interrogate 31 different biomarkers in liver samples at the single cell level. Specifically, we compared the liver immune microenvironment of mice within 2 different treatment groups (old mice on a high fat diet (HFD) and young mice on a normal diet (ND)) to the immune landscape of liver tumor tissue. Our goal is to identify distinct immune phenotypes present in both, liver tumor tissues and livers of HFD mice, compared to ND livers - to better understand how immune dysregulation can predispose an aged and obese liver to HCC. Citation Format: Adarsh Rajesh, Aaron Havas, Siva Karthik Varanasi, Katrina Evans, Bassem B. Cheikh, Clemens Duerrschmid, Najiba Mammadova, Peter Adams, Susan Kaech. a [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 2531.