Urinary Survivin mRNA Expression and Urinary Nuclear Matrix Protein 22 BladderChek® and Urine Cytology in the Detection of Transitional Cell Carcinoma of the Bladder

• Published in: Medical principles and practice 2012-01, Vol.21 (3), p.295-297
• Main Authors: Al-Maghrebi, May, Kehinde, Elijah O., Kapila, Kusum, Anim, Jehoram T.
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Antigens, Neoplasm - analysis; Antigens, Neoplasm - metabolism; Biomarkers, Tumor - analysis; Carcinoma, Transitional Cell - diagnosis; Carcinoma, Transitional Cell - pathology; Carcinoma, Transitional Cell - urine; Case-Control Studies; Chi-Square Distribution; Confidence Intervals; Cytodiagnosis; Feasibility Studies; Female; Humans; Male; Microtubule-Associated Proteins; Middle Aged; Nuclear Proteins - analysis; Nuclear Proteins - metabolism; Predictive Value of Tests; Real-Time Polymerase Chain Reaction; RNA, Messenger - biosynthesis; Sensitivity and Specificity; Short Communication; Urinary Bladder - pathology; Urinary Bladder Neoplasms - diagnosis; Urinary Bladder Neoplasms - pathology; Urinary Bladder Neoplasms - urine; Young Adult
• ISSN: 1011-7571; 1423-0151
• DOI: 10.1159/000334811

Objective: To compare the diagnostic performance of urine cytology (UC), survivin mRNA expression, and the NMP22 BladderChek® (NMP22BC) test for the detection, grading and staging of transitional cell carcinoma (TCC) of the bladder. Materials and Methods: Voided urine samples collected from 25 healthy controls and 80 patients diagnosed with TCC of the bladder were subjected to UC, the NMP22BC test and reverse-transcription real-time PCR for survivin mRNA expression. Results: Survivin mRNA expression showed the highest sensitivity (87.5%) followed by the NMP22BC test (61.3%) while UC exhibited the lowest sensitivity (40%). All three urine markers had a similar specificity of 96% (95% CI 80.5–99.3%). Survivin mRNA expression was the only urine marker that showed a significant difference in relation to tumour histological grade (χ 2 8.5, p = 0.015). None of the three urine markers was significantly related to tumour pathological stages. Conclusion: The diagnostic sensitivity of urinary survivin mRNA expression was superior to that of UC and the NMP22BC test and correlates with tumour pathological grade but not stage.