Effects of Renin-Angiotensin System Inhibitors on Renal Expression of Renalase in Sprague-Dawley Rats Fed With High Salt Diet

• Published in: Kidney & blood pressure research 2015-01, Vol.40 (6), p.605-613
• Main Authors: Wang, Yang, Xie, Bing-Qing, Gao, Wei-Hua, Yan, Ding-Yi, Zheng, Wen-Ling, Lv, Yong-Bo, Cao, Yu-Meng, Hu, Jia-Wen, Yuan, Zu-Yi, Mu, Jian-Jun
• Format: Article
• Language: English
• Subjects: Angiotensin II Type 1 Receptor Blockers - pharmacology; Angiotensin-Converting Enzyme Inhibitors - pharmacology; Animals; Blood Pressure - drug effects; Diet; Enalapril - pharmacology; Hydralazine - pharmacology; Kidney - drug effects; Kidney - enzymology; Male; Monoamine Oxidase - biosynthesis; Original Paper; Proteinuria; Rats; Rats, Sprague-Dawley; Receptor, Angiotensin, Type 1 - biosynthesis; Renalase; Renin - blood; Renin-angiotensin system; Renin-Angiotensin System - drug effects; Salt; Sodium Chloride, Dietary - adverse effects; Valsartan - pharmacology
• ISSN: 1420-4096; 1423-0143
• DOI: 10.1159/000368536

Background/Aims: The aim of our study was to investigate the effect of high-salt diet on the renal expression of renalase and the potential role of the local renin-angiotensin system in this process. Methods: Sprague-Dawley (SD) rats were divided into groups according to salt content in diet and drug treatment as follows: normal-salt diet (NS), high-salt diet (HS), high-salt intake with hydralazine (HS+H), high-salt diet with enalapril (HS+E), and high-salt diet with valsartan (HS+V). The dietary intervention and drugs were given for four weeks. Renin activity and angiotensin II type 1 receptor (AT1R) levels were detected by real-time PCR. Renalase mRNA and protein were also measured. Results: After four weeks, systolic blood pressure and proteinuria were significantly increased in the HS group with respect to the NS group. Dietary salt intake caused a dramatic decrease in renalase expression in the rat kidneys. Renal cortex renin and AT1R increased significantly in the HS and HS+H groups. Urinary protein was positively correlated with renal renin and AT1R levels. However, in the HS+E and HS+V groups, enalapril and valsartan failed to influence renal renalase expression but abolished the increase in proteinuria, renal cortex renin, and AT1R levels with respect to the HS group. Conclusion: This study indicates that high salt intake reduces renal expression, and renal RAS may be not involved in the regulation of renalase in SD rats fed with high-salt diet.