Prostaglandin-Like Substances Appearing in Canine Renal Venous Blood During Renal Ischemia: Their Partial Characterization By Pharmacologic And Chromatographic Procedures

• Published in: Circulation research 1970-11, Vol.27 (5), p.765-782
• Main Authors: McGIFF, JOHN C, CROWSHAW, KEITH, TERRAGNO, NORBERTO A, LONIGRO, ANDREW J, STRAND, JAMES C, WILLIAMSON, MARY ANNE, LEE, JAMES B, NG, KEVIN K. F
• Format: Article
• Language: English
• ISSN: 0009-7330; 1524-4571
• DOI: 10.1161/01.RES.27.5.765

Renal prostaglandins (PCs) might mediate an antihypertensive function of the kidney. The blood-superfused organ technique possesses the sensitivity (threshold < 0.4 ng/ml blood) and specificity required for identification of PGs in blood. Induction of unilateral renal ischemia in 14 chloraloseanesthetized dogs reduced renal blood flows from a mean value of 257 to 109 ml/min on the ischemic side and from 250 to 209 ml/min on the contralateral side. Concomitantly, PG-like substances were detected by assay organs in the venous blood of ischemic (13 experiments) and contralateral (11 experiments) kidneys. In one experiment, in a spontaneously hypertensive dog, PGs were not detected during renal ischemia.Renal venous blood and renal medullary tissue were extracted for acidic lipids and assayed for PG-like substances. Extracts of venous blood collected during renal ischemia and extracts of renal medulla yielded substances with biological activity indistinguishable from PG-like substances or PG standards. Chromatographic characterization of PG-like substances suggests that they are predominantly a mixture of PGE2 and PGF2α.