Impaired Contractile Performance of Cultured Rabbit Ventricular Myocytes After Adenoviral Gene Transfer of Na + -Ca 2+ Exchanger

Abstract —Na + -Ca 2+ exchanger (NCX) gene expression is increased in the failing human heart. We investigated the hypothesis that upregulation of NCX can induce depressed contractile performance. Overexpression of NCX was achieved in isolated rabbit ventricular myocytes through adenoviral gene tran...

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Published in:Circulation research 2000-09, Vol.87 (7), p.581-587
Main Authors: Schillinger, Wolfgang, Janssen, Paul M. L., Emami, Shahriyar, Henderson, Scott A., Ross, Robert S., Teucher, Nils, Zeitz, Oliver, Philipson, Kenneth D., Prestle, Jürgen, Hasenfuss, Gerd
Format: Article
Language:English
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Summary:Abstract —Na + -Ca 2+ exchanger (NCX) gene expression is increased in the failing human heart. We investigated the hypothesis that upregulation of NCX can induce depressed contractile performance. Overexpression of NCX was achieved in isolated rabbit ventricular myocytes through adenoviral gene transfer (Ad-NCX). After 48 hours, immunoblots revealed a virus dose-dependent increase in NCX protein. Adenoviral β-galactosidase transfection served as a control. The fractional shortening (FS) of electrically stimulated myocytes was analyzed. At 60 min −1 , FS was depressed by 15.6% in the Ad-NCX group (n=143) versus the control group (n=163, P
ISSN:0009-7330
1524-4571
DOI:10.1161/01.RES.87.7.581