Spatiotemporal Relationship Between Intracellular Ca 2+ Dynamics and Wave Fragmentation During Ventricular Fibrillation in Isolated Blood-Perfused Pig Hearts

Normal “master–slave” relationship between the action potential (AP) and intracellular Ca 2+ transient (Ca i T) is sometimes altered during ventricular fibrillation (VF). The nature of AP/Ca i T dissociation during VF and its role in inducing wavebreaks (WBs) remain unclear. We simultaneously mapped...

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Bibliographic Details
Published in:Circulation research 2007-10, Vol.101 (9)
Main Authors: Warren, Mark, Huizar, José F., Shvedko, Alexander G., Zaitsev, Alexey V.
Format: Article
Language:English
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Summary:Normal “master–slave” relationship between the action potential (AP) and intracellular Ca 2+ transient (Ca i T) is sometimes altered during ventricular fibrillation (VF). The nature of AP/Ca i T dissociation during VF and its role in inducing wavebreaks (WBs) remain unclear. We simultaneously mapped AP (RH237) and Ca i T (Rhod-2) during VF in blood-perfused pig hearts. We computed AP and Ca i T dominant frequency (DF) and Ca i T delay in each AP cycle. We identified WBs as singularity points in AP phase movies and sites of conduction block (CB) as sites where an AP wavefront failed to propagate. We analyzed spatiotemporal relationship between abnormal AP/Ca i T sequences and CB sites. We used a calcium chelator (BAPTA-AM) to abolish Ca i T and test its involvement in WB formation. During VF, the DF difference between AP and Ca i T was
ISSN:0009-7330
1524-4571
DOI:10.1161/CIRCRESAHA.107.162735