Targeted Deletion of Nuclear Factor κB p50 Enhances Cardiac Remodeling and Dysfunction Following Myocardial Infarction

• Published in: Circulation research 2009-03, Vol.104 (5), p.699-706
• Main Authors: Timmers, Leo, van Keulen, J Karlijn, Hoefer, Imo E, Meijs, Matthijs F.L, van Middelaar, Ben, den Ouden, Krista, van Echteld, Cees J.A, Pasterkamp, Gerard, de Kleijn, Dominique P.V
• Format: Article
• Language: English
• ISSN: 0009-7330; 1524-4571
• DOI: 10.1161/CIRCRESAHA.108.189746

Myocardial infarction is commonly complicated by left ventricular remodeling, a process that leads to cardiac dilatation, congestive heart failure and death. The innate immune system plays a pivotal role in the remodeling process via nuclear factor (NF)-κB activation. The NF-κB transcription factor family includes several subunits (p50, p52, p65, c-Rel, and Rel B) that respond to myocardial ischemia. The function of NF-κB p50, however, is controversial in this process. To clarify the role of NF-κB p50 in postinfarct left ventricular remodeling, myocardial infarction was induced in wild-type 129Bl6 mice and NF-κB p50–deficient mice. Without affecting infarct size, deletion of NF-κB p50 markedly increased the extent of expansive remodeling (end-diastolic volume176±13 μL versus 107±11 μL; P=0.003) and aggravated systolic dysfunction (left ventricular ejection fraction16.1±1.5% versus 24.7±3.7%; P=0.029) in a 28-day time period. Interstitial fibrosis and hypertrophy in the noninfarcted myocardium was increased in NF-κB p50 knockout mice. In the infarct area, a lower collagen density was observed, which was accompanied by an increased number of macrophages, higher gelatinase activity and increased inflammatory cytokine expression. In conclusion, targeted deletion of NF-κB p50 results in enhanced cardiac remodeling and functional deterioration following myocardial infarction by increasing matrix remodeling and inflammation.