Upregulation of Renal Sodium Transporters in D 5 Dopamine Receptor–Deficient Mice

• Published in: Hypertension (Dallas, Tex. 1979) Tex. 1979), 2010-06, Vol.55 (6), p.1431-1437
• Main Authors: Wang, Xiaoyan, Luo, Yingjin, Escano, Crisanto S., Yang, Zhiwei, Asico, Laureano, Li, Hewang, Jones, John E., Armando, Ines, Lu, Quansheng, Sibley, David R., Eisner, Gilbert M., Jose, Pedro A.
• Format: Article
• Language: English
• ISSN: 0194-911X; 1524-4563
• DOI: 10.1161/HYPERTENSIONAHA.109.148643

D 5 dopamine receptor (D 5 R)-deficient (D 5 −/− ) mice have hypertension that is aggravated by an increase in sodium intake. The present experiments were designed to test the hypothesis that a dysregulation of renal sodium transporters is related to the salt sensitivity in D 5 −/− mice. D 5 R was expressed in the renal proximal tubule, thick ascending limb, distal convoluted tubule, and cortical and outer medullary collecting ducts in D 5 +/+ mice. On a control Na + diet, renal protein expressions of NKCC2 (sodium-potassium-2 chloride cotransporter), sodium chloride cotransporter, and α and γ subunits of the epithelial sodium channel were greater in D 5 −/− than in D 5 +/+ mice. Renal renin abundance and urine aldosterone levels were similar but renal angiotensin II type 1 receptor (AT 1 R) protein expression was increased in D 5 −/− mice. An elevated Na + diet increased further the elevated blood pressure of D 5 −/− mice but did not affect the normal blood pressure of D 5 +/+ mice. The increased levels of NKCC2, sodium chloride cotransporter, and α and γ subunits of the epithelial sodium channel persisted with the elevated Na + diet and unaffected by chronic AT 1 R blockade (losartan) in D 5 −/− mice. The expressions of proximal sodium transporters NHE3 (sodium hydrogen exchanger type 3) and NaPi2 (sodium phosphate cotransporter type 2) were increased by the elevated Na + diet in D 5 −/− mice; the increased expression of NHE3 but not NaPi2 was abolished by AT 1 R blockade. Our findings suggest that the increased protein expression of sodium transporters/channels in distal nephron segments may be the direct consequence of the disruption of D 5 R, independent of the renin–angiotensin aldosterone system.