Abstract 18790: Therapeutic Efficacy of Cardiosphere-Derived Cells in a Transgenic Mouse Model of Nonischemic Dilated Cardiomyopathy

• Published in: Circulation (New York, N.Y.) N.Y.), 2013-11, Vol.128 (suppl_22)
• Main Authors: Aminzadeh, Mohammad A, Tseliou, Eleni, Sun, Baiming, Cheng, Ke, Malliaras, Konstantinos, Makkar, Raj R, Marbán, Eduardo
• Format: Article
• Language: English
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/circ.128.suppl_22.A18790

Abstract only Introduction: Cardiosphere-derived cells (CDCs) produce regenerative effects and improve function in the post-infarct setting. However, it is unclear whether CDCs are beneficial in non-ischemic dilated cardiomyopathy (DCM). We tested the effects of CDC transplantation in mice with cardiac-specific Gαq overexpression, which leads to intense myocardial oxidative/nitrosative stress and inflammation. These mice predictably develop progressive cardiac dilation and failure, with accelerated mortality. Methods and Results: A total of 71 mice were investigated in this study. Wild-type syngeneic mouse CDCs (10 5 cells total) or vehicle only were injected intramyocardially at five left ventricular sites in 6-, 8- and 11-week old Gαq mice. Cardiac function deteriorated in vehicle-treated mice over 3 months of follow-up, accompanied by oxidative stress, inflammation and adverse ventricular remodeling. Left ventricular (LV) ejection fraction and LV end-diastolic and end-systolic volumes were progressively worsened over 3 months observational period in vehicle-treated mice compared to CDC-treated group (p