Abstract 19049: MiR-126 Containing Exosomes From Human CD34+ Stem Cells Activate Endothelial Cells to Initiate Angiogenesis and Ischemic Tissue Repair

• Published in: Circulation (New York, N.Y.) N.Y.), 2013-11, Vol.128 (suppl_22)
• Main Authors: Sahoo, Susmita, Misener, Sol, Kim, David, Thorne, Tina, Vaughan, Douglas E, Losordo, Douglas W
• Format: Article
• Language: English
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/circ.128.suppl_22.A19049

Abstract only Introduction: Locally transplanted human CD34+ stem cells have been shown to improve exercise tolerance in patients with myocardial ischemia and promote angiogenesis in animal models. Recently we have demonstrated that CD34+ cells secrete membrane bound nano-vesicles called exosomes as a major component of their pro-angiogenic paracrine secretion. We hypothesize that cell-free exosomes from CD34+ cells (CD34 Exo) mimic the beneficial effects of the cells and promote angiogenesis and ischemic tissue repair via transfer of pro-angiogenic microRNAs, possibly to endothelial cells. Methods and Results: Therapeutic potential of CD34 Exo isolated from equal number of adult human peripheral blood-derived CD34+ cells was evaluated in an immunodeficient murine model of hind limb ischemia. Similar to cells, treatment with CD34 Exo resulted in significant improvement in ischemia compared to treatment with PBS (perfusion ratio: 1.01±0.04 v 0.57±0.1; capillary density: 1.8±0.3 v 0.9±0.1/HPF; limb amputation: 16% v 100%; p