Abstract 14422: Mitochondrial ROS Scavenger JP4-039 Improves Mitochondrial Oxidative Phosphorylation and Induces Angiogenesis in Murine and Human Coronary Artery and Atrial Endothelial Cells

• Published in: Circulation (New York, N.Y.) N.Y.), 2022-11, Vol.146 (Suppl_1), p.A14422-A14422
• Main Authors: Brinck Teixeira, Rayane, King, Michelle, Abedin, Taslova, Karbasiafshar, Catherine, Raj, Sidharth, Tucker, Aja, Sabra, Mohamed, Stanley, Madigan, Zhang, Peng, Wipf, Peter, Sellke, Frank W, Abid, Ruhul
• Format: Article
• Language: English
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/circ.146.suppl_1.14422

IntroductionReducing mitochondrial ROS (mito-ROS) has been a therapeutic goal to improve cardiovascular disease. Here, we evaluated the effects of mito-ROS scavenger JP4-039 with a focus on mouse heart and human coronary artery endothelial cell (MHEC, HCAEC). HypothesisWe hypothesized that JP4-039 would improve oxidative phosphorylation (OXPHOS) in EC and induce angiogenesis in post-AMI (acute myocardial infarction) hearts. MethodsHuman atrial explants, NOX2-overexpressing (OE) MHEC, and HCAEC were treated with 1-5μM JP4-039 and examined for sprouting, tube formation, mitochondrial complex expression (Western blot), and OXPHOS measurements (Seahorse). FVB mice (n=8/group) underwent AMI surgery and were assigned to vehicle (sunflower seed oil) or JP4-039 group (1mg/kg, i.p., 3x/week for 4 weeks). Left ventricle (LV) function and capillary density in infarcted LV were assessed by echocardiogram and immunofluorescence, respectively, 28 days after AMI. Data were analyzed by One-way ANOVA and Tukey’s post-hoc, or student’s t test, as appropriate. A p-value