Abstract 15734: Local Admixture Mapping of Metabolites in African Americans

• Published in: Circulation (New York, N.Y.) N.Y.), 2023-11, Vol.148 (Suppl_1)
• Main Authors: Cruz, Daniel E, Raffield, Laura M, Deng, Shuliang, Wilson, James, Gerszten, Robert E
• Format: Article
• Language: English
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/circ.148.suppl_1.15734

Abstract only Introduction: There is increasing evidence that metabolite levels in African Americans (AA) are influenced by population differentiated genetic variation. We investigated how local genetic ancestry may be associated with levels of circulating plasma metabolites using metabolomic profiling in the Jackson Heart Study, an AA cohort, in an attempt to identify additional genetic determinants of metabolite levels. Hypothesis: Certain metabolites will be associated with local African genetic ancestry. Methods: We performed admixture mapping using local ancestry estimates (probabilities of whether an individual has 0, 1 or 2 alleles of African ancestry at each site in the genome from RFMix, based on similarity to 1000G reference panel) from TOPMed sequencing data for autosomal SNPs. Associations were then tested for 302 targeted metabolites measured using LC-MS. Each local ancestry regression model was adjusted for age, sex, and estimated global ancestry. We used a previously established significance threshold for local African ancestry analysis, p