Lipids Stimulate the Production of 6-keto-prostaglandin F 1α in Human Dorsal Hand Veins

• Published in: Hypertension (Dallas, Tex. 1979) Tex. 1979), 2001-10, Vol.38 (4), p.858-863
• Main Authors: Haastrup, Adetola, Gadegbeku, Crystal A., Zhang, Da, Mukhin, Yurii V., Greene, Eddie L., Jaffa, Ayad A., Egan, Brent M.
• Format: Article
• Language: English
• ISSN: 0194-911X; 1524-4563
• DOI: 10.1161/hy1001.092615

Obese hypertensives have increased nonesterified fatty acids (NEFAs) and α-adrenergic vascular reactivity. Raising NEFAs locally with intralipid and heparin augments dorsal hand venoconstrictor responses to phenylephrine, an α 1 -adrenoceptor agonist. The enhanced venoconstrictor responses were reversed by indomethacin. The findings suggest that raising NEFAs leads to the generation of cyclooxygenase (COX) product(s) that enhance vascular reactivity. To test this notion, 6-keto-PGF 1α and TxB 2 , the stable metabolites of prostaglandin H 2 (PGH 2 ); prostacyclin (PGI 2 ); and thromboxane (TxA 2 ), were measured ≈1.5 to 2 cm downstream of a dorsal hand vein infusion of intralipid and heparin (n=10) or saline and heparin (n=5) for 2 hours each. During the third hour, intralipid and heparin (experimental) and saline and heparin (control) were continued, and either saline (control) or indomethacin (intervention) were infused. Intralipid and heparin raised local 6-keto PGF 1α concentrations by 350% to 500% ( P