Effects of Antisense Oligodeoxynucleotide Targeting of the α 2B -Adrenergic Receptor Messenger RNA in the Central Nervous System

The results of previous studies with genetically engineered mice have suggested that an intact central α 2B -adrenergic receptor (α 2B -AR) subtype mediates the development and maintenance of salt-induced hypertension. In the present study, we sought to further define the role of this receptor by in...

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Bibliographic Details
Published in:Hypertension (Dallas, Tex. 1979) Tex. 1979), 2001-11, Vol.38 (5), p.1075-1080
Main Authors: Kintsurashvili, Ekaterina, Gavras, Irene, Johns, Conrado, Gavras, Haralambos
Format: Article
Language:English
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Summary:The results of previous studies with genetically engineered mice have suggested that an intact central α 2B -adrenergic receptor (α 2B -AR) subtype mediates the development and maintenance of salt-induced hypertension. In the present study, we sought to further define the role of this receptor by injecting antisense oligodeoxynucleotides (AS-ODNs), targeting a selected sequence of the α 2B -AR mRNA, into the lateral cerebral ventricle of rats that had undergone prior subtotal nephrectomy and dietary salt loading. Cell culture studies showed that these AS-ODNs could block α 2B -AR protein generation. Before AS-ODN injection, blood pressure (BP) averaged 133±5 mm Hg during the daytime and rose to 165±4 mm Hg during the nighttime activity hours ( P
ISSN:0194-911X
1524-4563
DOI:10.1161/hy1101.093426