Abstract 315: Endothelial And Autonomic Dysfunctions Induced By High Fructose Intake Are Modulated By The Angiotensin Converting Enzyme (ACE) Gene Dosage

• Published in: Hypertension (Dallas, Tex. 1979) Tex. 1979), 2014-09, Vol.64 (suppl_1)
• Main Authors: Moraes-Silva, Ivana C, Souza, Leandro E, Irigoyen, Maria-Claudia, Casarini, Dulce E
• Format: Article
• Language: English
• ISSN: 0194-911X; 1524-4563
• DOI: 10.1161/hyp.64.suppl_1.315

Abstract only ACE gene polymorphism may be associated with different responses to physiological and pathological stimuli. Therefore, we tested whether low, normal or high levels of ACE gene dosage influence on the effects of chronic and excessive fructose intake (F). Male mice (22±1g) harboring 1, 2 or 3 copies of the ACE gene received F (100g/l) or tap water for 8 weeks. After this period, glucose tolerance (0-120 min after glucose load=1.5 g/Kg ip) and lipids were measured; blood pressure (BP) signals were recorded (Windaq, 4kHz), and BP variability was analyzed in frequency domain (FFT). After euthanasia, mesenteric resistance artery (MRA) was dissected out and rings were mounted in an isometric myograph (DMT,620M). MRA responses to noradrenaline (Nor; 0.1nM-1μM) and acetylcholine (Ach; 0.01nM-30μM) were studied. One-way ANOVA and p