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Abstract P162: In vivo miR-431 Inhibition Protects Against Vascular Damage and Hypertension

Abstract only Background: Vascular injury is an early manifestation of hypertension. microRNAs (miRNAs) play an important role in cardiovascular disease, but their implication in vascular injury remains unclear. Using small and total RNA sequencing, we identified in murine mesenteric arteries (MAs)...

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Published in:Hypertension (Dallas, Tex. 1979) Tex. 1979), 2017-09, Vol.70 (suppl_1)
Main Authors: Huo, Ku-Geng, Fraulob-Aquino, Julio C., Barhoumi, Tlili, Richer, Chantal, Coelho, Suellen C., Ouerd, Sofiane, Caillon, Antoine, Lajoie, Mathieu, Sinnett, Daniel, Paradis, Pierre, Schiffrin, Ernesto L.
Format: Article
Language:English
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Summary:Abstract only Background: Vascular injury is an early manifestation of hypertension. microRNAs (miRNAs) play an important role in cardiovascular disease, but their implication in vascular injury remains unclear. Using small and total RNA sequencing, we identified in murine mesenteric arteries (MAs) a conserved angiotensin (Ang) II-upregulated Dlk1-Dio3 miRNA miR-431 that correlated with blood pressure (BP), and an Ang II-downregulated BP-correlated conserved putative miR-431 target, the transcriptional factor ETS homologous factor ( Ehf ). miR-431 might be involved in vascular remodeling as Ehf regulates expression of extracellular matrix genes including alpha-1 type I collagen ( Col1a1 ) and of other Dlk1-Dio3 miRNAs. In this study, we proposed to validate the miR-431- Ehf - Col1a1 interaction in vitro and in vivo , and determine whether miR-431 inhibition antagonizes angiotensin (Ang) II-induced hypertension and vascular injury. Methods and Results: Transfection of miR-431 mimics into human aortic smooth muscle cells decreased Ehf expression (0.13±0.05 fold, P
ISSN:0194-911X
1524-4563
DOI:10.1161/hyp.70.suppl_1.p162