Abstract 095: Renal Transcriptomic Analysis of Transgenic Mice Containing Human Cyp11b2 Gene

• Published in: Hypertension (Dallas, Tex. 1979) Tex. 1979), 2018-09, Vol.72 (Suppl_1)
• Main Authors: Mopidevi, Brahmaraju, Sivankutty, Indu, Perla, Sravan, Jain, Sudhir, Kumar, Ashok
• Format: Article
• Language: English
• ISSN: 0194-911X; 1524-4563
• DOI: 10.1161/hyp.72.suppl_1.095

Abstract only The hCYP11B2 gene encodes aldosterone synthase, the rate-limiting enzyme in the biosynthesis of aldosterone. Inappropriate excess levels of aldosterone induce positive sodium balance and predisposes to hypertension and other cardiovascular problems. Epidemiological studies have suggested that the variant -344T of the Cyp11B2 gene is associated with hypertension and myocardial hypertrophy. We have found that hCyp11B2 gene has three SNPs in 1 Kb of its promoter that are in complete linkage disequilibirium. These SNPs are T/C at -344, C/T at -470 and C/A at -663. Thus variant -344T always occurs with variants -470C, -663A (Hap-I), and variant -344C almost always occurs with variants, -470T, -663T (Hap-II). We have generated novel transgenic (TG) mice with the hCYP11B2 gene, targeted to the mHPRT locus, with either haplotype I or II variants. We observed increased baseline blood pressure in Hap I TG mice (Hap-I 117±2.5 vs. Hap-II 109±1.9 mm Hg, p