Abstract 038: The Arg16 / Gln27 Diplotype of the Beta-2 Adrenergic Receptor: Cardiovascular Insights from a Novel Transgenic Mouse Model

• Published in: Hypertension (Dallas, Tex. 1979) Tex. 1979), 2019-09, Vol.74 (Suppl_1)
• Main Authors: Treesaranuwattana, Thitinan, Maris, Stephen, Garza, Amanda E, Brooks, Danielle, Pojoga, Luminita H
• Format: Article
• Language: English
• ISSN: 0194-911X; 1524-4563
• DOI: 10.1161/hyp.74.suppl_1.038

Abstract only The beta-2 adrenergic receptor (B2AR) is an established regulator of vascular tone and cardiac contractility. Two polymorphisms of the B2AR (Gly16Arg and Glu27Gln) have been associated with cardiovascular phenotypes, including hypertension, salt sensitivity of BP, cardiovascular disease and responses to antihypertensives. These same variants are associated with changes in receptor downregulation and desensitization in pulmonary tissues; however, there is no information on the effect of these polymorphisms on B2AR expression/function in cardiovascular tissues. To investigate the effect of these variants on cardiovascular function, we used the CRISPR/Cas9 approach to generate transgenic mice that carry the diplotype Arg16/Gln27 on 2, 1 or 0 alleles, i. e. mutant (Mut), heterozygous (Het) and wild-type (WT). Both male and female mice displayed significantly higher body weights with the increase in number of mutated alleles (P