Abstract P150: Genes of a Brown Norway Chromosome 2 Fragment Introgressed Into Hypertensive Dahl Salt-Sensitive Background Exert Pro-Inflammatory Effects When Stimulated by a High-Salt Diet

• Published in: Hypertension (Dallas, Tex. 1979) Tex. 1979), 2019-09, Vol.74 (Suppl_1)
• Main Authors: Berillo, Olga, Ouerd, Sofiane, Huo, Ku-Geng, Rehman, Asia, Richer, Chantal, Sinnett, Daniel, Kwitek, Anne E, Paradis, Pierre, Schiffrin, Ernesto L
• Format: Article
• Language: English
• ISSN: 0194-911X; 1524-4563
• DOI: 10.1161/hyp.74.suppl_1.P150

Abstract only Background: Chromosome 2 (Chr2) introgression from normotensive Brown Norway (BN) rats into hypertensive Dahl salt-sensitive (SS) background (consomic S B 2) reduced blood pressure (BP) and vascular inflammation under normal-salt diet (NSD). We hypothesized that BN Chr2 contains anti-inflammatory genes that could reduce BP elevation and vascular inflammation in rats fed NSD and high-salt diet (HSD). Method: Four- to 6-week old male SS and congenic rats containing the BN Chr2 distal portion (S B 2a) and middle segment (S B 2b) were fed NSD or HSD (4% NaCl) for 8 weeks. We determined systolic BP (SBP) by telemetry, reactive oxygen species (ROS) generation using dihydroethidium staining, and vascular cell adhesion molecule 1 (VCAM-1), monocyte chemoattractant protein-1 (MCP-1) expression and monocyte/macrophage (MoMϕ) infiltration by immunofluorescence in aorta or perivascular fat (PVAT). RNA was extracted from aorta and used for small and total RNA sequencing and data were analyzed using a systems biology approach. Differentially expressed genes (DEGs) were identified with fold change >1.3 and fold discovery rate