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Abstract 470: Gata4, Mef2c and Tbx5 More Efficiently Transdifferentiate Endothelial Cells into Cardiomyocyte-like Cells Through Endothelial-Mesenchymal Transition Process

Abstract only Background: The cardiac transcription factors Gata4, Mef2c, and Tbx5 (GMT) have been used to successfully reprogram cardiac fibroblasts into induced cardiomyocyte-like cells (iCMs). However their effects on other cell types remain largely unknown. Endothelial cells are an important pop...

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Bibliographic Details
Published in:Circulation research 2018-08, Vol.123 (Suppl_1)
Main Authors: Sanagasetti, Deepthi, Singh, Vivek P, Pinnamaneni, Jaya Pratap, Pugazenthi, Aarthi, Jang, Anna, So, Davis, Kim, Mary, Yang, Lina, Wang, Kai, Yang, Jianchang, Mathison, Megumi, Rosengart, Todd K
Format: Article
Language:English
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Summary:Abstract only Background: The cardiac transcription factors Gata4, Mef2c, and Tbx5 (GMT) have been used to successfully reprogram cardiac fibroblasts into induced cardiomyocyte-like cells (iCMs). However their effects on other cell types remain largely unknown. Endothelial cells are an important population of the heart by Pinto et al. (2016). Given the more profound iCM induction effects observed in vivo, we have speculated that reprogramming of endogenous endothelial cells may also play an important role. Purpose: To investigate the cellular reprograming effects and mechanisms of GMT factors on cardiac endothelial cells and compare the outcomes with that from cardiac fibroblasts. Methods: Adult rat cardiac endothelial cells (REC) and cardiac fibroblasts (RCF) were purchased commercially. The cells were cultured in appropriate media and infected with GMT-expressing lentivirus and maintained with iCM media. Fourteen days later Immunofluorescence staining, FACS, and qRT-PCR were performed. Results: Both GMT treated RCFs and RECs showed positive staining of cardiac marker cTnT and αActinin-positive cells via immunofluorescence staining. FACS analysis showed GMT treated RECs had 16.7% of cTnT positive cells versus 3.3% in GMT treated RCF’s. qRT-PCR results demonstrated significant up-regulation of cTnT in GMT infected RECs (74.4±23.3 times, p
ISSN:0009-7330
1524-4571
DOI:10.1161/res.123.suppl_1.470