Abstract WP148: ApoA-I Mimetic Peptide Reduces Vascular and White Matter Damage After Stroke in Type 2 Diabetic Mice

• Published in: Stroke (1970) 2019-02, Vol.50 (Suppl_1)
• Main Authors: Wang, Xiaohui, Li, Rongwen, Zacharek, Alex, Landschoot-Ward, Julie, Chopp, Michael, Chen, Jieli, Cui, Xu
• Format: Article
• Language: English
• ISSN: 0039-2499; 1524-4628
• DOI: 10.1161/str.50.suppl_1.WP148

Abstract only Diabetes mellitus leads to a higher risk of ischemic stroke and worse functional outcome compared to the general population. We investigate whether L-4F, an economical apolipoprotein A-I mimetic peptide, reduces neurovascular and white matter damage in a clinically relevant stroke model using type-2 diabetic (T2DM) db/db mice. Methods: Adult male db/db mice were subjected to 2h middle cerebral artery occlusion (MCAo) and were orally administered saline (n=52) or L-4F (16mg/kg, n=34) with treatment initiated 2h after stroke and continued daily until sacrifice 4 days after MCAo. Mouse-brain endothelial cells, primary arterial cells derived from the aorta of C57BL/6 mice 1 day after MCAo, and primary cortical neurons derived from E15 C57BL/6-embryos were employed. These cells were subjected to high glucose conditions to simulate diabetes. L-4F treatment of T2DM-stroke mice exhibited reduced hemorrhage/infarct volume and mortality rate, as well as improved neurological functional outcome 4 days after MCAo compared with vehicle control T2DM-stroke mice (p