Abstract P784: A Novel Rat Model of Embolic Cerebral Ischemia Using a Cell-Implantable Radiopaque Hydrogel Micro Fiber

• Published in: Stroke (1970) 2021-03, Vol.52 (Suppl_1), p.AP784-AP784
• Main Authors: Komatsu, Teppei, Ohta, Hiroki, Takakura, Naoki, Kitagawa, Tomomichi, Hata, Junichi, Terawaki, Koshiro, Koizumi, Makoto, Muta, Kanako, Kurashina, Yuta, Onoe, Hiroaki, Okano, Hirotaka J, Iguchi, Yasuyuki
• Format: Article
• Language: English
• ISSN: 0039-2499; 1524-4628
• DOI: 10.1161/str.52.suppl_1.P784

IntroductionThe failure of neuroprotective treatment-related clinical trials may be partially caused by unestablished animal models. We aimed to develop a novel focal stroke model using a cell-implantable radiopaque hydrogel micro fiber. The micro fiber is made of barium alginate hydrogel containing zirconia, and fabricated in a dual coaxial laminar flow microfluidic device. MethodsUsing male Sprague Dawley rats (n=10), a catheter (ID 0.42mm, OD 0.55mm) was navigated from the caudal ventral artery to the left internal carotid artery using digital subtraction angiography. A radiopaque hydrogel micro fiber (0.4 mm in diameter, 1 mm in length) was advanced in the catheter by slow injection of heparinized physiological saline to establish local occlusion. 9.4-T MRI and 2% 2,3,5-triphenyl tetrazolium chloride staining were performed 24 hours after the creation of the stroke model. ResultsThe anterior cerebral artery-middle cerebral artery bifurcation was selectively embolized at all rats. The median operating time was 8.5 min (interquartile range; 6.25-12 min). The median infarct volume (mm3) was 262 (interquartile range; 260-274) 24 hours after occlusion. ConclusionsWe present a novel rat model for focal infarct only in the middle cerebral artery territory using a radiopaque hydrogel micro fiber. Furthermore, this model can be used for regeneration research of cerebral infarction because the hydrogel micro fiber can encapsulate stem cell.Figure Legends APuncture of the caudal ventral artery in a rat. BCerebral angiography of a rat. COcclusion of the anterior cerebral artery-middle cerebral artery bifurcation by a radiopaque hydrogel fiber (arrowhead). DMR angiography detected selective occlusion of left middle cerebral artery. ET2-weighted image showed infarct only in the middle cerebral artery after 24 hours occlusion. A radiopaque fiber did not affect the 9.4-T MRI. FRadiopaque hydrogel fiber encapsulated HeLa cells.