Core-shell microspheres by dispersion polymerization as promising delivery systems for proteins

Functional poly(methyl methacrylate) core-shell microspheres were prepared by dispersion polymerization. An appropriate selection of experimental parameters and in particular of the initiator and stabilizer amount and of the medium solvency power allowed a monodisperse sample as large as 600 nm to b...

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Bibliographic Details
Published in:Journal of biomaterials science. Polymer ed. 2005-01, Vol.16 (12), p.1557-1574
Main Authors: Sparnacci, Katia, Laus, Michele, Tondelli, Luisa, Bernardi, Cinzia, Magnani, Laura, Corticelli, Franco, Marchisio, Marco, Ensoli, Barbara, Castaldello, Arianna, Caputo, Antonella
Format: Article
Language:English
Subjects:
Acrylic Resins - chemistry
Adsorption
Animals
Biocompatible Materials - chemistry
Cattle
Chemical Phenomena
Chemistry, Physical
CORE-SHELL MICROSPHERES
DELIVERY
DISPERSION POLYMERIZATION
Drug Carriers
Feasibility Studies
Female
Fluorescein
Fluorescent Dyes
HeLa Cells
Humans
Hydrogen-Ion Concentration
Indoles
Injections, Intramuscular
Isoelectric Point
Mice
Mice, Inbred Strains
Microscopy, Confocal
Microscopy, Electron, Scanning
Microspheres
Molecular Structure
Molecular Weight
Nuclear Magnetic Resonance, Biomolecular
Particle Size
Polymethyl Methacrylate - chemistry
PROTEIN
Proteins - chemistry
Proteins - pharmacokinetics
Serum Albumin, Bovine - pharmacokinetics
Surface Properties
Time Factors
Trypsin - chemistry
Trypsin - pharmacokinetics
VACCINE
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Summary:Functional poly(methyl methacrylate) core-shell microspheres were prepared by dispersion polymerization. An appropriate selection of experimental parameters and in particular of the initiator and stabilizer amount and of the medium solvency power allowed a monodisperse sample as large as 600 nm to be prepared. To this purpose, low initiator concentration, high steric stabilizer amount and a low solvency power medium were employed. The microspheres present a core-shell structure in which the outer shell is constituted by the steric stabilizer which affords carboxylic groups able to interact with basic proteins, such as trypsin, whose adsorption is essentially driven by the carboxylic group density in the microsphere shell. Finally, fluorescent microspheres were prepared for biodistribution studies and shown to be readily taken up by the cells both in vitro and in vivo. These results suggest that these microspheres are promising delivery systems for the development of novel protein-based vaccines.
ISSN:0920-5063
1568-5624
DOI:10.1163/156856205774576673