Examining binding capacity of aptamers based on fluorescence image segmentation

In order to meet the needs of verifying binding capacities in aptamer selection experiments, a binding capacity examination device for aptamers and targets based on the fluorescence image segmentation was developed in this paper. The fluorescence imaging module is used to collect images of aptamers...

Full description

Saved in:
Bibliographic Details
Published in:Materials express 2022-08, Vol.12 (8), p.1049-1058
Main Authors: Ji, Minjie, Wang, Chao, Jin, Baijiang, Guo, Zhukang, Chen, Zhu, Chen, Hui, Li, Song, Deng, Yan, Zhang, Yuanying, He, Nongyue
Format: Article
Language:English
Subjects:
Aptamer
Binding Capacity Examination
Fluorescence Imaging Segmentation
Selection Process Monitoring
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to meet the needs of verifying binding capacities in aptamer selection experiments, a binding capacity examination device for aptamers and targets based on the fluorescence image segmentation was developed in this paper. The fluorescence imaging module is used to collect images of aptamers binding to target cells, and then the average fluorescence intensity of a single cell is calculated using methods of the cell image segmentation and the fluorescence image registration. By comparing the fluorescence intensity, the binding capacity of aptamers can be investigated and the progress of selection experiments can be monitored. The application value of this paper is to provide a new method for the dynamic monitoring of the aptamer binding performance during the automatic selection.
ISSN:2158-5849
DOI:10.1166/mex.2022.2252