A Cholinergic Agonist Attenuates Endotoxin-Induced Uveitis in Rats

• Published in: Investigative ophthalmology & visual science 2007-06, Vol.48 (6), p.2719-2725
• Main Authors: Chi, Zai-Long, Hayasaka, Seiji, Zhang, Xue-Yun, Cui, Hu-Shan, Hayasaka, Yoriko
• Format: Article
• Language: English
• Subjects: alpha7 Nicotinic Acetylcholine Receptor; Animals; Aqueous Humor - cytology; Aqueous Humor - metabolism; Biological and medical sciences; Ciliary Body - metabolism; Cytokines - genetics; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Eye and associated structures. Visual pathways and centers. Vision; Fundamental and applied biological sciences. Psychology; Injections, Intraperitoneal; Iris - metabolism; Lipopolysaccharides - toxicity; Medical sciences; Nicotine - pharmacology; Nicotinic Agonists - pharmacology; Ophthalmology; Rats; Rats, Wistar; Receptors, Nicotinic - genetics; Receptors, Nicotinic - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Uvea diseases; Uveitis - chemically induced; Uveitis - metabolism; Uveitis - prevention & control; Vertebrates: nervous system and sense organs
• ISSN: 0146-0404; 1552-5783; 1552-5783
• DOI: 10.1167/iovs.06-0644

Investigation of physiological anti-inflammatory mechanisms can contribute to the treatment of inflammatory disorders. The purpose of the present study was to investigate the effect of nicotine, a selective cholinergic agonist, on endotoxin-induced uveitis (EIU) in rats and the underlying molecular mechanism. Lipopolysaccharide (LPS; endotoxin) and nicotine were injected intraperitoneally. Clinical scores were evaluated by slit lamp. Intracameral protein content and the number of cells were determined. Immunohistochemical reactivity of alpha7 nicotine acetylcholine receptor (alpha7nAChR) was examined in the iris and ciliary body (ICB). mRNA and protein levels of cytokines and chemokines were measured by real-time PCR and enzyme-linked immunosorbent assay. After LPS injection, clinical scores, as well as protein content and number of cells in the aqueous humor increased during 18 to 36 hours. Nicotine inhibited the endotoxin-induced elevation of these levels. mRNA and protein of alpha7nAChR expression levels were significantly increased by LPS and/or nicotine injection. Nicotine showed no effects on endotoxin-induced elevation of mRNA levels in ICB. However, nicotine decreased the endotoxin-induced elevation of interleukin (IL)-6, IL-1beta, tumor necrosis factor (TNF)-alpha, cytokine-induced neutrophil chemoattractant (CINC)-1, and monocyte chemotactic protein (MCP)-1, but did not affect IL-10 in the serum and aqueous humor. Nicotine attenuated endotoxin-induced uveitis through directly decreasing the levels of multiple cytokines and chemokines in the aqueous humor, but did not affect the mRNA levels of these factors. The findings suggest that the nicotinic anti-inflammatory pathway may be involved in the pathogenesis of EIU.