Characterization of a Specific Antibody to the Rat Angiotensin II AT 1 Receptor

We raised a polyclonal antibody against a decapeptide corresponding to the carboxyl terminus of the rat angiotensin II AT 1 receptor. This antibody was demonstrated to be specific for the rat receptor according to a number of approaches. These included (a) the ultrastructural localization of immunog...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1999-04, Vol.47 (4), p.507-515
Main Authors: Giles, Michelle E., Fernley, Ross T., Nakamura, Yoshio, Moeller, Ingrid, Aldred, G. Peter, Ferraro, Tania, Penschow, Jennifer D., McKinley, Michael J., Oldfield, Brian J.
Format: Article
Language:English
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Summary:We raised a polyclonal antibody against a decapeptide corresponding to the carboxyl terminus of the rat angiotensin II AT 1 receptor. This antibody was demonstrated to be specific for the rat receptor according to a number of approaches. These included (a) the ultrastructural localization of immunogold-labeled receptor on the surfaces of zona glomerulosa cells in the adrenal cortex, (b) the specific labeling of Chinese hamster ovarian (CHO) cells transfected with AT 1 receptors, (c) the identification of a specific band on Western blots, (d) the immunocytochemical co-localization of angiotensin receptors on neurons in the lamina terminalis of the brain shown to be responsive to circulating angiotensin II, as shown by the expression of c-fos, and (e) the correlation between the expression of the mRNA of the AT 1 receptor and AT 1 receptor immunoreactivity.
ISSN:0022-1554
1551-5044
DOI:10.1177/002215549904700409