Cerium-based Histochemical Demonstration of Oxidative Stress in Taurocholate-induced Acute Pancreatitis in Rats: A Confocal Laser Scanning Microscopic Study

Direct in vivo histological detection of oxygen-derived free radicals (OFRs) in inflammatory conditions is not fully resolved. We report an application of cerium histochemistry (in which capture of OFRs by Ce atoms results in laser-reflectant cerium-perhydroxide precipitates) combined with reflectan...

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Published in:The journal of histochemistry and cytochemistry 1999-09, Vol.47 (9), p.1201-1212
Main Authors: Telek, Geza, Scoazec, Jean-Yves, Chariot, Jacques, Ducroc, Robert, Feldmann, Gerard, Roze, Claude
Format: Article
Language:English
Subjects:
Acute Disease
Animals
Cerium - metabolism
Histocytochemistry - methods
Hydroxides
Immunohistochemistry
Leukocytes - drug effects
Leukocytes - metabolism
Male
Microscopy, Confocal
Oxidative Stress
Pancreatitis - chemically induced
Pancreatitis - metabolism
Pancreatitis - pathology
Peroxides - metabolism
Platelet Endothelial Cell Adhesion Molecule-1 - biosynthesis
Rats
Rats, Wistar
Reactive Oxygen Species - metabolism
Taurocholic Acid
Tetradecanoylphorbol Acetate - pharmacology
Time Factors
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Summary:Direct in vivo histological detection of oxygen-derived free radicals (OFRs) in inflammatory conditions is not fully resolved. We report an application of cerium histochemistry (in which capture of OFRs by Ce atoms results in laser-reflectant cerium-perhydroxide precipitates) combined with reflectance confocal laser scanning microscopy (CLSM) to demonstrate the evolution of oxidative stress in taurocholate-induced acute pancreatitis (AP) in rats. Animals were perfused with CeCl3 in vivo and cryostat sections of pancreata were studied by CLSM. Vascular endothelium was immunolabeled for PECAM-1. OFR production by isolated polymorphonuclear leukocytes (PMNs) incubated in vitro with CeCl3 was quantified by image analysis. In the pancreas, strong OFR-derived cerium reflectance signals were seen in acinar cells at 1-2 hr, capillaries and small venules were frequently engorged by cerium precipitates, and adherent PMNs presented weak intracellular reflectance signals. At 8-24 hr, acinar cell OFR production decreased, whereas adherent/transmigrated PMNs displayed abundant intra- and pericellular reflectance. PECAM-1 expression was unchanged. PMNs from ascites or blood showed significant (p
ISSN:0022-1554
1551-5044
DOI:10.1177/002215549904700912