Increased heat shock protein 70 expression following toxicant-mediated cytotoxicity : A ubiquitous marker of toxicant exposure?

The up-regulation of heat shock protein (HSP) expression has been proposed as a general biomarker of cellular protection against various environmental stresses and chemicals. The present study investigated the possibility of using HSP70 up-regulation as a biomarker of toxicant exposure in vitro. Cel...

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Bibliographic Details
Published in:Alternatives to laboratory animals 2005-04, Vol.33 (2), p.105-110
Main Authors: FARZANEH, Parivash, ALLAMEH, Abdolamir, PRATT, Steven, MOORE, Nicholas, TRAVIS, Lucy, GOTTSCHALG, Elke, KIND, Clive, FRY, Jeffrey
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biomarkers - metabolism
Carcinoma, Hepatocellular - metabolism
Cell Line, Tumor - drug effects
Cell Line, Tumor - metabolism
Cell Survival - drug effects
Chemical and industrial products toxicology. Toxic occupational diseases
Dose-Response Relationship, Drug
HSP70 Heat-Shock Proteins - metabolism
Liver Neoplasms - metabolism
Medical sciences
Metals and various inorganic compounds
Neutral Red - metabolism
Pesticides, fertilizers and other agrochemicals toxicology
Rats
Toxicology
Up-Regulation - drug effects
Xenobiotics - classification
Xenobiotics - toxicity
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Summary:The up-regulation of heat shock protein (HSP) expression has been proposed as a general biomarker of cellular protection against various environmental stresses and chemicals. The present study investigated the possibility of using HSP70 up-regulation as a biomarker of toxicant exposure in vitro. Cells of a rat hepatoma cell line (FGC4) were exposed to concentrations of 1,3-dichloroacetone, duroquinone, diquat dibromide, menadione, hydrogen peroxide, cadmium chloride (CdCl2) and sodium (meta)arsenite (NaAsO2) that elicited 20-50% cytotoxicity over a 24-hour period, and HSP70 levels were measured by ELISA. Up-regulation of HSP70 expression was demonstrated following treatment with menadione, CdCl2 and NaAsO2, but not with the other chemicals tested. A shorter exposure time (6 hours) and/or the use of non-toxic concentrations reduced the level of HSP70 up-regulation with menadione, CdCl2 and NaAsO2, but did not uncover any up-regulation with the other chemicals. Although the toxicity of the majority of the chemicals tested is believed to involve an oxidative stress component, the results of this study clearly demonstrate that up-regulation of HSP70 expression cannot be used as a general biomarker of toxicant exposure in vitro.
ISSN:0261-1929
2632-3559
DOI:10.1177/026119290503300205