FRAME alternatives laboratory database. 1. In vitro basal cytotoxicity determined by the Kenacid blue total protein assay

A database of over 280 chemicals has been compiled by using a mouse 3T3-L1 fibroblast-like cell line in exponential growth, exposed to chemicals for 72 hours in a 96-well tissue culture plate format, and determining cell number via the Kenacid blue (KB) assay for total protein. Ranking the chemicals...

Full description

Saved in:
Bibliographic Details
Published in:Alternatives to laboratory animals 2006-05, Vol.34 (2), p.151-175
Main Authors: Clothier, R, Gottschalg, E, Casati, S, Balls, M
Format: Article
Language:English
Subjects:
3T3 Cells - drug effects
3T3 Cells - metabolism
3T3 Cells - pathology
Animal Testing Alternatives
animal use alternatives
Animals
bioassays
Biological and medical sciences
cell lines
cell proliferation
Cell Survival - drug effects
chemical substances
cytotoxicity
Databases, Factual
Dose-Response Relationship, Drug
Humans
in vitro studies
Medical sciences
Mice
Neutral Red
Organic Chemicals
protein synthesis
Proteins - metabolism
toxicity testing
Toxicity Tests - methods
Toxicology
Xenobiotics - classification
Xenobiotics - toxicity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A database of over 280 chemicals has been compiled by using a mouse 3T3-L1 fibroblast-like cell line in exponential growth, exposed to chemicals for 72 hours in a 96-well tissue culture plate format, and determining cell number via the Kenacid blue (KB) assay for total protein. Ranking the chemicals according to their basal cytotoxicity, expressed as the concentration (mM) that inhibits increase in total cellular protein over 72 hours by 50% (the ID50 value) shows a wide range of ID50 values, from 0.00003 mM to 10,096 mM. This information includes the results for MEIC chemicals 1-50, and we have now added basal cytotoxicity data for 23 of the next 25 MEIC chemicals. When the neutral red uptake (NRU) assay was performed with the same cell cultures, before the KB assay, very similar indications of basal cytotoxicity were obtained. Comparisons between the results with 3T3-L1 cells and with a human fibroblast-like cell line, BCL-D1 showed a significant difference in order of magnitude of the ID50 value for only 5 of 52 chemicals. However, there was a difference in ID50 value of more than one order of magnitude for 8 of 24 chemicals tested with an undifferentiated teratocarcinoma cell line, F9.
ISSN:0261-1929
2632-3559
DOI:10.1177/026119290603400203