Phage Display Screening of Epithelial Cell Monolayers Treated with EGTA: Identification of Peptide FDFWITP that Modulates Tight Junction Activity

• Published in: SLAS discovery 2007-12, Vol.12 (8), p.1092-1101
• Main Authors: Herman, Richard E., Makienko, Ekaterina G., Prieve, Mary G., Fuller, Mark, Houston, JR, Michael E., Johnson, Paul H.
• Format: Article
• Language: English
• Subjects: calcium chelation; claudin; epithelial barrier; paracellular ion permeation; peptide phage display; tight junction
• ISSN: 2472-5552; 2472-5560
• DOI: 10.1177/1087057107310216

Phage display was used to screen for peptides that modulate the activity of epithelial cell tight junctions. Panning with a phage library that displays random 7-mers was performed using monolayers of human bronchial epithelial cells (16HBE14o–) treated with a calcium chelator, ethylene glycol-bis(2-aminoethylether)-N, N, N′, N′-tetraacetic acid (EGTA), to increase accessibility to the junctional complex/paracellular space, followed by subtractive panning. A novel peptide, FDFWITP, identified as a potential tight junction modulator, was synthesized in linear and cyclic forms with lysine residues added to improve solubility. The cyclic form of the peptide reduced transepithelial electrical resistance (TER) in a concentration-dependent manner (80% reduction at 100 μM and 95% reduction at 500 μM) and was reversible within 2 h; the linear form only affected TER at the highest concentration. Interestingly, the constrained peptide did not increase permeation of the model small molecule, fluorescein. The highly selective activity of FDFWITP supports the hypothesis that ions and small molecules may be transported paracellularly across tight junctions by separate pathways.