Development of an Automated Culture System for Laboratory Evolution

Laboratory evolution provides phenotype–genotype mappings and quantitative analysis of selective pressures, giving important insights about evolutionary dynamics. Moreover, parallel laboratory evolution clarifies which phenotypic and genotypic changes are inevitable for adaptive evolution. Such para...

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Bibliographic Details
Published in:Journal of Laboratory Automation 2014-10, Vol.19 (5), p.478-482
Main Authors: Horinouchi, Takaaki, Minamoto, Teruaki, Suzuki, Shingo, Shimizu, Hiroshi, Furusawa, Chikara
Format: Article
Language:English
Subjects:
automated culture system
Escherichia coli
laboratory evolution
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Summary:Laboratory evolution provides phenotype–genotype mappings and quantitative analysis of selective pressures, giving important insights about evolutionary dynamics. Moreover, parallel laboratory evolution clarifies which phenotypic and genotypic changes are inevitable for adaptive evolution. Such parallel experiments, however, remain labor-intensive. In this study, to facilitate massive parallel laboratory evolution, we developed an automated culture system that can maintain hundreds of independent culture series in exponential growth phase under various culture conditions. We demonstrate the performance of this automated culture system using the laboratory evolution of Escherichia coli under various stressors.
ISSN:2472-6303
2211-0682
2472-6311
1540-2452
DOI:10.1177/2211068214521417