Hesperidin, a flavanone glycoside, on lipid peroxidation and antioxidant status in experimental myocardial ischemic rats

• Published in: Redox report : communications in free radical research 2010-10, Vol.15 (5), p.217-223
• Main Authors: Selvaraj, Palanisamy, Pugalendi, Kodukkur Viswanathan
• Format: Article
• Language: English
• Subjects: Alanine Transaminase - blood; Animals; ANTIOXIDANTS; Antioxidants - metabolism; Aspartate Aminotransferases - blood; Cardiotonic Agents - pharmacology; Catalase - metabolism; Creatine Kinase - blood; Glutathione Peroxidase - metabolism; Glutathione Reductase - metabolism; Glutathione Transferase - metabolism; HESPERIDIN; Hesperidin - chemistry; Hesperidin - pharmacology; Hesperidin - therapeutic use; ISOPROTERENOL; Isoproterenol - pharmacology; L-Lactate Dehydrogenase - blood; LIPID PEROXIDATION; Lipid Peroxidation - drug effects; Lipid Peroxides - metabolism; Male; Molecular Structure; MYOCARDIAL ISCHEMIA; Myocardial Ischemia - chemically induced; Myocardial Ischemia - drug therapy; Myocardial Ischemia - metabolism; Myocardium - cytology; Myocardium - metabolism; Myocardium - pathology; Random Allocation; Rats; Rats, Wistar; Superoxide Dismutase - metabolism; Thiobarbituric Acid Reactive Substances - metabolism; Troponin I - blood; Troponin T - blood
• ISSN: 1351-0002; 1743-2928
• DOI: 10.1179/135100010X12826446921509

Myocardial infarction continues to be a leading cause of mortality world-wide. Novel therapies are needed to treat the myocardial ischemia. This study was undertaken to evaluate the cardioprotective role of hesperidin on isoproterenol-induced myocardial ischemia in rats. Myocardial ischemia was induced by subcutaneous injection of isoproterenol hydrochloride (85 mg/kg body weight), for two consecutive days. Isoproterenol-administered rats showed elevated levels of cardiac markers (aspartate transaminase, alanine transaminase, lactate dehydrogenase, creatine kinase, creatine kinase-MB, cardiac troponins T and I) when compared with control and hesperidin treatment groups (100, 200 and 400 mg/kg body weight). The serum levels of cardiac markers were significantly reduced at the doses of 200 mg and 400 mg. All further experiments were carried out at the 200 mg dose. Lipid peroxidation markers (thiobarbituric acid reactive substances, lipid hydroperoxides and conjugated dienes) were elevated significantly in the plasma and heart whereas non-enzymic antioxidants (vitamin C, vitamin E and reduced glutathione) were decreased significantly. Activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferease and glutathione reductase declined significantly in the heart of ischemic rats. However, after hesperidin treatment, all the above parameters reverted to normal levels. This study demonstrated that the cardioprotective effect of hesperidin on ischemic rats could be due to its antilipidperoxidative and antioxidant properties.