Traumatic brain injury accelerates kindling epileptogenesis in rats

Objectives: Traumatic brain injury (TBI) is a well-known cause of symptomatic epilepsy. In animal models of post-traumatic epilepsy (PTE), progression of trauma to epilepsy takes several weeks to months. Although this long process is similar to clinical PTE, it is costly and laborious. We used a com...

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Bibliographic Details
Published in:Neurological research (New York) 2016-03, Vol.38 (3), p.269-274
Main Authors: Eslami, Mansoureh, Ghanbari, Elham, Sayyah, Mohammad, Etemadi, Fatemeh, Choopani, Samira, Soleimani, Mansoureh, Amiri, Zohreh, Hadjighassem, Mahmoudreza
Format: Article
Language:English
Subjects:
Amygdala - physiopathology
Amygdala kindling
Animals
Brain Injuries, Traumatic - complications
Brain Injuries, Traumatic - etiology
Cerebral Cortex - pathology
Controlled cortical impact
Convulsants - toxicity
Disease Models, Animal
Electric Stimulation - adverse effects
Epilepsy - etiology
Epileptogenesis
Kindling, Neurologic - physiology
Male
Parieto-temporal cortex
Pentylenetetrazole - toxicity
PTZ kindling
Rats
Rats, Wistar
Statistics, Nonparametric
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Summary:Objectives: Traumatic brain injury (TBI) is a well-known cause of symptomatic epilepsy. In animal models of post-traumatic epilepsy (PTE), progression of trauma to epilepsy takes several weeks to months. Although this long process is similar to clinical PTE, it is costly and laborious. We used a combination of TBI and kindling as an accelerated animal model to develop epilepsy in much shorter period compared to that occurring in PTE. Methods: Traumatic brain injury was exerted to parieto-temporal cortex of anaesthetised rats by controlled cortical impact (CCI, 5 mm round tip, 4.5 mm/seconds velocity and 150 ms duration). Chemical kindling started 24 hours after CCI by intraperitoneal injection of 30 mg/kg pentylenetetrazole (PTZ) every other day until manifestation of three consecutive generalised seizures. Rapid electrical kindling of the amygdala began 1 week after TBI by exertion of 12 daily threshold stimuli (50 Hz mono-phasic square-wave stimulus of 1 ms per wave for 3 seconds) with 5 minutes interval between each stimulation until the rats became kindled. Results: Controlled cortical impact injury accelerated rate of both chemical and electrical kindling. Number of PTZ injections required for acquisition of generalised seizures decreased from 13.1 ± 1.6 in sham-operated animals to 7.1 ± 0.3 in traumatic rats (p 
ISSN:0161-6412
1743-1328
DOI:10.1179/1743132815Y.0000000086