Ultrastructural Localization of Diagnostically Relevant Melanoma-Specific Antibodies by Immunoelectron Microscopy

Postembedding immunoelectron microscopy can be used for the detection of antigenic sites in ultrathin sections. The protein A-gold technique, when used in conjunction with tissue dehydration at low temperature followed by embedding in a hydrophilic resin, can provide a satisfactory means for assessi...

Full description

Saved in:
Bibliographic Details
Published in:Journal of histotechnology 2003-12, Vol.26 (4), p.263-269
Main Authors: Taatjes, Douglas J., Pang, Yijun, Turkovich, Michele von, DeLance, Nicole, Cooper, Kumarasen
Format: Article
Language:English
Subjects:
HMB-45
immunocytochemistry
MART-1
melanocyte
melanoma
protein A-gold technique
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Postembedding immunoelectron microscopy can be used for the detection of antigenic sites in ultrathin sections. The protein A-gold technique, when used in conjunction with tissue dehydration at low temperature followed by embedding in a hydrophilic resin, can provide a satisfactory means for assessing the ultrastructural localization of diagnostically relevant antigens. In this brief article, we will demonstrate the usefulness of this technique to demonstrate the subcellular localization of the binding sites for the melanoma-associated antibodies HMB-45 and MART-1. (The J Histotechnol 26:263, 2003)
ISSN:0147-8885
2046-0236
DOI:10.1179/his.2003.26.4.263