A Protocol for the Staining of Cement Lines in Adult Human Bone Using Toluidine Blue

Increased definition of cement lines aids in the identification of secondary osteons in bone. A staining protocol taken from Burr (Anat Rec 232:180-189, 1991) and modified by Havill using toluidine blue on plastic-embedded bone specimens was applied to cranial core and femoral shaft sections but pro...

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Bibliographic Details
Published in:Journal of histotechnology 2005-06, Vol.28 (2), p.73-79
Main Authors: Osborne, Daniel L., Curtis, Janene
Format: Article
Language:English
Subjects:
bone histology
celnent lines
plastic embedding
stain
toluidine blue
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Summary:Increased definition of cement lines aids in the identification of secondary osteons in bone. A staining protocol taken from Burr (Anat Rec 232:180-189, 1991) and modified by Havill using toluidine blue on plastic-embedded bone specimens was applied to cranial core and femoral shaft sections but proved to be too time-consuming and inconsistent. Experimentation resulted in a different protocol for each bone type, perhaps as the result of differences in section thickness and cortical area. Staining of femoral sections proved particularly difficult, with increased etching duration and agitation proving most influential in intensity and uniforlnity of stain. (The J Histotechnol 28:73, 2005) Submitted August 20, 2004; accepted with revisions April 15, 2005
ISSN:0147-8885
2046-0236
DOI:10.1179/his.2005.28.2.73