Dendritic cell-derived IL-2 production is regulated by IL-15 in humans and in mice

Dendritic cells (DCs) are involved in the initiation and regulation of innate and adaptive immune responses. Several molecular mechanisms regulate these diverse DC functions, and we have previously reported that mouse dendritic cells (mDCs) can produce interleukin-2 (IL-2) in vitro and in vivo, in r...

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Bibliographic Details
Published in:Blood 2005-01, Vol.105 (2), p.697-702
Main Authors: Feau, Sonia, Facchinetti, Valeria, Granucci, Francesca, Citterio, Stefania, Jarrossay, David, Seresini, Samantha, Protti, Maria Pia, Lanzavecchia, Antonio, Ricciardi-Castagnoli, Paola
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
CD40 Antigens - metabolism
Cells, Cultured
Dendritic Cells - cytology
Dendritic Cells - drug effects
Dendritic Cells - metabolism
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunobiology
Interleukin-15 - genetics
Interleukin-15 - metabolism
Interleukin-15 - pharmacology
Interleukin-2 - genetics
Interleukin-2 - metabolism
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Modulation of the immune response (stimulation, suppression)
Monocytes - cytology
RNA, Messenger - analysis
Signal Transduction - immunology
Specific Pathogen-Free Organisms
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Summary:Dendritic cells (DCs) are involved in the initiation and regulation of innate and adaptive immune responses. Several molecular mechanisms regulate these diverse DC functions, and we have previously reported that mouse dendritic cells (mDCs) can produce interleukin-2 (IL-2) in vitro and in vivo, in response to microbial activation and T-cell-mediated stimuli. This property is shared by different DC subtypes, including Langerhans cells. Here we show that, on appropriate stimulation, human DCs, both plasmacytoid and myeloid subtypes, also express IL-2. Interestingly, the production of IL-2 by myeloid DCs is induced by T-cell-mediated stimuli and depends on the presence of IL-15. The key role of this cytokine in regulating IL-2 production was also confirmed in the mouse system. In particular, we could show that DCs from IL-15-deficient mice were strongly impaired in the ability to produce IL-2 after interactions with different microbial stimuli. Our results indicate that DC-produced IL-2 is tightly coregulated with the expression of IL-15.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood-2004-03-1059