Differences between Peripheral and Bone Marrow Lipidomics in Patients with Severe Aplastic Anemia and Its Finding in Predicting the Early Immunosuppressive Therapy Response

• Published in: Blood 2023-11, Vol.142 (Supplement 1), p.2718-2718
• Main Authors: Sun, Zexing, Hong, Yilei, Jiang, Wanzhi, Shen, Yingying, Hu, Huijin, Hu, Tonglin, Liu, Qi, Zhuang, Zhenchao, Zhou, Yuhong, Ye, Baodong, Wu, Dijiong
• Format: Article
• Language: English
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood-2023-184631

Aplastic anemia (AA) is a common clinical hematological disease, which is characterized by pancytopenia due to bone marrow hematopoietic failure. AA can be divided into severe AA (SAA) and non-severe AA (NSAA) based on disease severity. Anti-thymoglobulin (ATG)-based immunosuppressive treatment (IST) is recommended as the main first-line treatment for AA patients when stem cell transplantation is not available. The mechanism of AA has not been fully clarified. When considering role of the bone marrow microenvironment, current studies showed that the abnormal of quantity, function, and differentiation of mesenchymal stem cells (MSCs) were involved in the occurrence and development of AA, including the boost of adipocyte. Han, et al. [PMID: 35445952] found that the peripheral serum lipid profile may have great meaning in the differentiation diagnosis between transfusion-dependent non-severe AA and hypo- myelodysplastic syndrome (MDS), as well as in predicting their response to cyclosporine (CsA). Our previous study [PMID: 36192750] also revealed that the higher apolipoprotein-A is a potential prognostic biomarker for severe AA patients treated with ATG-based IST. However, the understanding of peripheral serum lipid metabolism may not fully represent the changes in bone marrow. Herein, we try to investigate the difference of lipid metabolomic profile between bone marrow and peripheral serum in SAA patients, and explore its meaning in predicting early IST response. In this study, a total of 11 SAA patients and 15 age and sex matched healthy donors were enrolled. Among them, six SAA received ATG based IST (Table. 1). An orthogonal partial least-squares discrimination analysis (OPLS-DA) model was established to analyze the differences between the subgroups and overfitting was accessed by permutation test (Fig A, B). The following volcano map (Fig. C) and heat map (Fig. D) confirmed that there were differences in subgroups to a certain degree. To explain the difference, we firstly compared lipid metabolism profile between patients (P) and donors (D) in peripheral serum (ps) (P ps Vs D ps) and bone marrow (bm) serum (P bm Vs D bm), and found that the difference in bone marrow was more distinct than that in peripheral serum, which suggested bone marrow may be more reliable as clinical samples in studies (Fig. E). Secondly, bone marrow serum and peripheral serum (P bm Vs P ps) in SAA patients were compared, fatty acids were upregulated (Fig. F), which indicated that