Human Cytomegalovirus Inhibits Granulopoiesis Via Direct Infection and Inducing Apoptosis

Human cytomegalovirus (HCMV) infection can cause delayed leukocyte recovery after bone marrow transplantation and often associated with suppression of granulocyte/macrophage progenitor. Leukocyte lineage may be one of the major sites of HCMV infection. However, whether HCMV can interfere with CFU-GM...

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Bibliographic Details
Published in:Blood 2005-11, Vol.106 (11), p.3859-3859
Main Authors: Wang, Qing Wen, He, Zheng Xian, Li, Xiao Feng, Xiong, Wei, Chen, Mei Lian, Li, Karen Kwai Har, Yang, Mo
Format: Article
Language:English
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Summary:Human cytomegalovirus (HCMV) infection can cause delayed leukocyte recovery after bone marrow transplantation and often associated with suppression of granulocyte/macrophage progenitor. Leukocyte lineage may be one of the major sites of HCMV infection. However, whether HCMV can interfere with CFU-GM formation and induce apoptosis in granulocyte/macrophage progenitor have not been well investigated. Human bone marrow mononuclear cells (Ficoll), promyelocyte cell line HL-60 and HCMV AD169 strain were co-cultured. Each bone marrow specimen was HCMV DNA and HCMV IgM negative by PCR and ELISA test. Our results showed that HCMV significantly inhibited the formation of CFU-GM as shown in two different concentrations of viral infection groups: 139.26 ± 5.42 (2×105pfu/ml), and 124.19 ± 8.82 (2×106pfu/ml) (colonies/2×105cells/ml, n=26). These were significant differences compared with the blank control group (P
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V106.11.3859.3859