SAGE Analysis Demonstrates Increased Expression of TOSO Contributing to Fas Mediated Resistance in CLL

The molecular pathogenesis of CLL remains unclear; therefore we sought to identify new genes involved in the pathogenesis or mechanisms of resistance in CLL and other B cell malignancies. To this end we performed SAGE in CLL cells and compared results with those from healthy B cells (nCD19+). Gene e...

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Bibliographic Details
Published in:Blood 2007-11, Vol.110 (11), p.1121-1121
Main Authors: Proto-Siqueira, Rodrigo, Panepucci, Rodrigo A., Careta, Francisco P., Rizzatti, Edgar G., Lee, Abigail, Clear, Andrew, Morris, Kelly, Falcão, Roberto P., Zago, Marco A., Gribben, John G.
Format: Article
Language:English
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Summary:The molecular pathogenesis of CLL remains unclear; therefore we sought to identify new genes involved in the pathogenesis or mechanisms of resistance in CLL and other B cell malignancies. To this end we performed SAGE in CLL cells and compared results with those from healthy B cells (nCD19+). Gene expression profiles were obtained by SAGE and individual gene expression was measured by real time PCR. Analysis revealed only 27 genes with significant (p< 0.001) differences between mutated and unmutated subtypes of CLL cells. In contrast to the high level of similarity between the CLL subtypes, comparison of the complete CLL SAGE libraries versus nCD19, revealed that 56 genes were over represented and 50 genes were down regulated in CLL (fold change higher than 20x, p
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V110.11.1121.1121